Genetic markers for search of rhizobia based on symbiotic genes

The possible application of rhizobial symbiotic genes as markers for the search and primary identification of rhizobia from temperate-zone legumes was studied. It was shown that conservative sym genes nifH and nifD could be used as markers for rapid search of rhizobia among the analyzed isolates, wh...

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Bibliographic Details
Published in:	Microbiology (New York) Vol. 86; no. 5; pp. 640 - 646
Main Authors:	Akimova, E. S., Gumenko, R. S., Vershinina, Z. R., Baymiev, Al. Kh, Baymiev, An. Kh
Format:	Journal Article
Language:	English
Published:	Moscow Pleiades Publishing 01-09-2017 Springer Nature B.V
Subjects:	Astragalus cicer Biomedical and Life Sciences Experimental Articles Genetic markers Legumes Life Sciences Medical Microbiology Microbiology NifH gene Onobrychis arenaria Root nodules rhizobia legumes symbiotic genes
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Summary:	The possible application of rhizobial symbiotic genes as markers for the search and primary identification of rhizobia from temperate-zone legumes was studied. It was shown that conservative sym genes nifH and nifD could be used as markers for rapid search of rhizobia among the analyzed isolates, while more variable genes nifK and nodC could be used for their primary identification. Efficiency of the proposed method was shown in analysis of bacterial isolates obtained from Onobrychis arenaria and Astragalus cicer root nodules.
ISSN:	0026-2617 1608-3237
DOI:	10.1134/S0026261717050034