Evaluation of Antioxidant Activities and Antifungal Activity of Different Plants Species Against Pink Mold Rot-Causing Trichothecium roseum

• Published in: Arabian journal for science and engineering (2011) Vol. 42; no. 6; pp. 2279 - 2289
• Main Authors: Balkan, Bilal, Balkan, Seda, Aydoğdu, Halide, Güler, Necmettin, Ersoy, Hüseyin, Aşkın, Buket
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01-06-2017; Springer Nature B.V
• Subjects: Antioxidants; Engineering; Food; Fungicides; Gallic acid; Germination; Humanities and Social Sciences; Mold; multidisciplinary; Oregano; Phenols; Plant diseases; Research Article - Biological Sciences; Science; Tomatoes; Vegetables; SEM; Antifungal; Antioxidant; Pink mold rot
• ISSN: 2193-567X; 1319-8025; 2191-4281
• DOI: 10.1007/s13369-017-2484-4

Trichothecium roseum
causes the pink mold rot in many fruits and vegetables around the world. Due to this infection, significant losses arise in foods. In order to control this infection, plant extracts offer alternative treatment for fungicides. In this study, 50 plant species were screened for their antifungal effects against
T. roseum
.
Anthemis arvensis, Origanum vulgare, Sambucus ebulus
and
Thymus longicaulis
powders totally inhibited the mycelia growth of
T. roseum
at 10% (w/v). The powders of
Chelidonium majus
and
Clinopodium vulgare
were effective to
T. roseum
, with a percentage of inhibition of mycelia growth higher than 70%. MIC of
A. arvensis
aqueous extracts were lower than the other extracts (125 
μ
g
/ml). Also its extracts inhibited the spore germination by 100% at 1000 
μ
g
/ml. The incidence of the pink mold rot on tomatoes which were treated with
C. majus
aqueous extracts (75, 150 and 300 mg/ml) was lower than the extracts of other plants when compared to control. At concentration of 300 mg/ml,
C. majus
extracts prevented the disease by 71.42%. By the SEM, it was determined at the 4MIC extracts, cell wall degradation, swelling, flattening, lysis, collapsing and wrinkling on the hyphal structure. The highest total phenolic and flavanol contents were observed in
O. vulgare
extracts (310.49 mg GA/g) and
T. longicaulis
(5.24 mg CE/g). The
EC
50
values of the experimented extracts were lowered than the
EC
50
value of Gallic acid (1.87 mg/ml). Meanwhile, in all of the extracts there were phenolic compounds, protocatechuic, chlorogenic, caffeic acid and kaempferol as determined with HPLC system. This research demonstrates that
C. majus
aqueous extracts may possess high potential to control the pink mold rot on tomatoes as new natural antifungal products.