Marker-assisted improvement of bacterial blight resistance in parental lines of Pusa RH10, a superfine grain aromatic rice hybrid

• Published in: Molecular breeding Vol. 26; no. 2; pp. 293 - 305
• Main Authors: Basavaraj, S. H., Singh, Vikas K., Singh, Atul, Singh, Ashutosh, Singh, Anita, Anand, Deepti, Yadav, Sheel, Ellur, Ranjith K., Singh, Devinder, Gopala Krishnan, S., Nagarajan, M., Mohapatra, T., Prabhu, K. V., Singh, Ashok K.
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Netherlands 01-08-2010; Springer Nature B.V
• Subjects: Biomedical and Life Sciences; Biotechnology; Blight; Fertility; Genes; Genomes; Grain; Life Sciences; Marker-assisted selection; Markers; Molecular biology; Oryza; Plant biology; Plant Genetics and Genomics; Plant Pathology; Plant Physiology; Plant Sciences; Rice; Ultrafines; Foreground selection; Gene pyramiding; Marker-assisted selection; Rice; Bacterial blight; Background selection
• ISSN: 1380-3743; 1572-9788
• DOI: 10.1007/s11032-010-9407-3

Pusa RH10, the widely cultivated superfine grain aromatic rice hybrid, and its parental lines Pusa6B and PRR78 are susceptible to bacterial blight (BB) disease caused by
Xanthomonas oryzae
pv.
oryzae
. Pusa1460, a Basmati rice variety, was utilized as the donor for introgressing BB resistance genes
xa13
and
Xa21
into Pusa6B and PRR78 using a marker-assisted backcross breeding program. The markers RG136 and pTA248 linked to BB resistance genes
xa13
and
Xa21
, respectively, were used for foreground selection. Seventy-four STMS markers polymorphic between Pusa6B and Pusa1460, and 54 STMS markers polymorphic between PRR78 and Pusa1460, were utilized for background selection to recover the recurrent parent genome ranging from 85.14 to 97.30% and 87.04 to 92.81% in the 10 best BC
2
F
5
families of Pusa6B and PRR78, respectively. RM6100, an STMS marker linked to fertility restorer gene (
Rf
), was used for marker-assisted selection of
Rf
gene in an improved version of PRR78. The extent of donor segments in the improved version of Pusa6B was estimated to be <0.97 and <2.15 Mb in the genomic regions flanking
xa13
and
Xa21
, respectively, whereas in improved PRR78, it was estimated to be <2.07 and <3.45 Mb in the corresponding genomic regions. Improved lines of Pusa6B and PRR78 showed yield advantages of up to 8.24 and 5.23%, respectively. The performance of the BB-resistant version of Pusa RH10 produced by intercrossing the improved parental lines was on a par with or superior to the original Pusa RH10.