Nuclear Magnetic Resonance and Infrared Spectroscopy Examination of Blood for Diagnosis of the Diffuse Hepatopathy State

• Published in: Optics and spectroscopy Vol. 128; no. 6; pp. 787 - 793
• Main Authors: Kruchinina, M. V., Kruchinin, V. N., Shuvalov, G. V., Minin, I. V., Minin, O. V.
• Format: Journal Article
• Language: English
• Published: Moscow Pleiades Publishing 01-06-2020; Springer Nature B.V
• Subjects: Adenosine; Adenosine diphosphate; Adenosine monophosphate; Adenosine triphosphate; Biophotonics; Diagnostic systems; Erythrocytes; Fibrosis; Infrared spectroscopy; Lasers; Liver; Molecular structure; NMR; NMR spectroscopy; Nuclear magnetic resonance; Optical Devices; Optics; Phosphates; Photonics; Physics; Physics and Astronomy; Sensitivity; Spectrum analysis; P NMR spectroscopy; erythrocytes; diffuse hepatopathy; degree of fibrosis; IR spectroscopy; blood serum
• ISSN: 0030-400X; 1562-6911
• DOI: 10.1134/S0030400X20060120

67 middle-aged (48 ± 1 years) patients with diffuse liver disease of various origins have been examined. It has been established that an increase in the degree of fibrosis is associated with an increase in the resonance intensity of glucose-6-phosphates, adenosine monophosphate (AMP), inosine monophosphate (IMP), pyrophosphates, and 2,3-bisphosphoglyceric acid (BPG) and a decrease in the resonances of inorganic phosphates and phosphocreatine, γ-, α-, and β-AMP, uridine triphosphate (UTP), Hb- and Mg-adenosine triphosphate (ATP), and β- and α- adenosine diphosphate (ADP). The predictive value of a positive result of
31
P NMR spectroscopy of erythrocytes for liver fibrosis is 92.3% (the sensitivity is 85.71% and the specificity is 80%). In the IR spectra of the blood serum of patients with severe fibrosis, in contrast to the cases of moderate fibrosis, the resonance intensity is higher at 1280 and 3190 cm
‒1
and significantly lower at 776, 818, 889, 966, 1399, 1450, 1570, and 1635 cm
‒1
, which indicates the presence of protein molecules with a secondary structure mainly in the form of a spiral (α helix), in contrast to the structure in the form of a folded sheet (β sheet) at
p
< 0.001‒0.05. The combined use of
31
P NMR spectroscopy of erythrocyte suspensions and IR spectroscopy of blood serum made it possible to increase the diagnostic accuracy (96%), sensitivity (97%), and specificity (91%) in distinguishing the degrees of liver fibrosis as compared with the separate use of these techniques.