Production and immunochemical characterization of monoclonal antibody to IRR ectodomain

• Published in: Russian journal of bioorganic chemistry Vol. 43; no. 6; pp. 653 - 657
• Main Authors: Mozhaev, A. A., Erokhina, T. N., Serova, O. V., Deyev, I. E., Petrenko, A. G.
• Format: Journal Article
• Language: English
• Published: Moscow Pleiades Publishing 01-11-2017; Springer Nature B.V
• Subjects: Biochemistry; Biomedical and Life Sciences; Biomedicine; Bioorganic Chemistry; Insulin; Life Sciences; Monoclonal antibodies; Organic Chemistry; Pancreas; Stomach; insulin receptor-related receptor; monoclonal antibodies
• ISSN: 1068-1620; 1608-330X
• DOI: 10.1134/S1068162017060097

The insulin receptor-related receptor (IRR) is the only known metabotropic sensor of extracellular alkaline medium involved in the regulation of the acid–base balance in the body. IRR is expressed in certain cell populations of the kidney, stomach, and pancreas that can come into contact with the extracellular fluids with alkaline pH. To study IRR structure and function, we obtained a stable hybridoma cell-line-producing antibody to the extracellular portion of the receptor. The monoclonal antibody isolated from ascitic fluids showed a positive reaction with the antigen in the ELISA test. The minimum working concentration of antibodies was 12.5 ng/mL. The ability of the antibodies to specifically recognize the purified ectodomain of IRR and the fulllength receptor was confirmed by western blot, immunoprecipitation, and immunocytochemistry.