Mapping QTLs of resistance to head splitting in cabbage (Brassica oleracea L.var. capitata L.)

• Published in: Molecular breeding Vol. 35; no. 5; pp. 1 - 12
• Main Authors: Pang, Wenxing, Li, Xiaonan, Choi, Su Ryun, Nguyen, Van Dan, Dhandapani, Vignesh, Kim, Yoon-Young, Ramchiary, Nirala, Kim, Jin Gyu, Edwards, David, Batley, Jacqueline, Na, Jonghyun, Kim, HyeRan, Lim, Yong Pyo
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Netherlands 01-05-2015; Springer Nature B.V
• Subjects: Biomedical and Life Sciences; Biotechnology; Brassica; Brassica oleracea; Cell density; Cell size; Cell walls; Chromosome 1; Chromosome 2; Chromosomes; Field emission microscopy; Gene mapping; Homology; Inbreeding; Karyotypes; Life Sciences; Mapping; Markers; Molecular biology; Plant biology; Plant Genetics and Genomics; Plant Pathology; Plant Physiology; Plant Sciences; Quantitative trait loci; Scanning electron microscopy; Splitting; Synteny; Quantitative trait loci; Head-splitting resistance; Cabbage; Genetic map; Scanning electron microscope; Bulked segregant analysis
• ISSN: 1380-3743; 1572-9788
• DOI: 10.1007/s11032-015-0318-1

Cabbage head splitting can greatly affect both the quality and commercial value of cabbage (
Brassica oleracea
). To detect the genetic basis of head-splitting resistance, a genetic map was constructed using an F
2
population derived by crossing “748” (head-splitting-resistant inbred line) and “747” (head-splitting-susceptible inbred line). The map spans 830.9 cM and comprises 270 markers distributed in nine linkage groups, which correspond to the nine chromosomes of
B. oleracea
. The average distance between adjacent markers was 3.6 cM. A total of six quantitative trait loci (QTLs) conferring resistance to head splitting were detected in chromosome 2, 4, and 6. Two QTLs,
SPL
-
2
-
1
and
SPL
-
4
-
1
, on chromosomes 2 and 4, respectively, were detected in the experiments over 2 years, suggesting that these two potential loci were important for governing the head-splitting resistance trait. Markers BRPGM0676 and BRMS137, which were tightly linked with head-splitting resistance, were detected in the conserved QTL
SPL
-
2
-
1
region using bulked segregant analysis. Synteny analysis showed that
SPL
-
2
-
1
was anchored to a 3.18-Mb genomic region of the
B. oleracea
genome, homologous to crucifer ancestral karyotype E block in chromosome 1 of
Arabidopsis thaliana.
Moreover, using a field emission scanning electron microscope, significant differences were observed between the two parental lines in terms of cell structures. Line “747” had thinner cell wall, lower cell density, larger cell size, and anomalous cell wall structure compared with the resistant line “748.” The different cell structures can provide a cytological base for assessing cabbage head splitting.