sFRP1 Expression Induces miRNAs That Modulate Wnt Signaling in Chronic Myeloid Leukemia Cells

• Published in: Molecular biology (New York) Vol. 54; no. 4; pp. 563 - 569
• Main Authors: Pehlivan, M., Soyoz, M., Cerci, B., Coven, H. I. K., Yuce, Z., Sercan, H. O.
• Format: Journal Article
• Language: English
• Published: Moscow Pleiades Publishing 01-07-2020; Springer Nature B.V
• Subjects: Abl protein; BCR protein; Biochemistry; Biomedical and Life Sciences; Chromosome 5; Chronic myeloid leukemia; Frizzled-related protein 1; Fusion protein; Hematopoietic stem cells; Human Genetics; Leukemia; Leukocytes (granulocytic); Life Sciences; Macrophages; Molecular Cell Biology; Myeloid leukemia; Philadelphia chromosome; Protein-tyrosine kinase; Stem cells; Wnt protein; chronic myeloid leukemia; miRNA; secreted Frizzled related protein 1
• ISSN: 0026-8933; 1608-3245
• DOI: 10.1134/S0026893320040135

Chronic myeloid leukemia is a clonal hematopoietic stem cell disease characterized by myeloid expansion. The hallmark of the disease is the Philadelphia chromosome, which results in the formation of the
BCR-ABL
oncogene, a tyrosine kinase that is involved in many signaling pathways including Wnt signaling. The latter has a unique role in chronic myeloid leukemia progression; activated signaling leads to the establishment of an additional leukemic stem cell population derived from granulocyte-macrophage progenitors. sFRP1 is an inhibitor of Wnt signaling and its expression is important for differentiation and maintenance of hematopoietic stem cells. In this study, we aimed to investigate miRNAs that target Wnt signaling by being co-induced along with the expression of sFRP1 in K562 cells. We present a detailed analysis of hsa-mir-221-3p, hsa-mir-222-3p, hsa-mir-106b-5p, hsa-let-7f-5p, hsa-mir-182-5p, hsa-mir-191-5p, and hsa-mir-183-5p and their target genes and their involvement in Wnt signaling.