Substrate Specificity of Methanogenic Communities from Lake Baikal Bottom Sediments Associated with Hydrocarbon Gas Discharge

• Published in: Microbiology (New York) Vol. 87; no. 4; pp. 549 - 558
• Main Authors: Bukin, S. V., Pavlova, O. N., Kalmychkov, G. V., Ivanov, V. G., Pogodaeva, T. V., Galach’yants, Yu. P., Bukin, Yu. S., Khabuev, A. V., Zemskaya, T. I.
• Format: Journal Article
• Language: English
• Published: Moscow Pleiades Publishing 01-07-2018; Springer Nature B.V
• Subjects: Acetic acid; Biomedical and Life Sciences; Carbon dioxide; Culture media; Experimental Articles; Life Sciences; Medical Microbiology; Methane; Methanogenic archaea; Microbiology; Pore water; Sediments; Sodium acetate; Substrate specificity; Volcanoes; Lake Baikal; methanogenic archaea; methane; microbial communities; bottom sediments; Lake Baikal; high-throughput sequencing
• ISSN: 0026-2617; 1608-3237
• DOI: 10.1134/S0026261718040045

Methane production by microbial communities from Lake Baikal bottom sediments with different chemical composition of pore water was studied. Methane production was more active in the media supplemented with H
2
: CO
2
and H
2
+ CH
3
COONa, rather than on media with acetate as the sole source of carbon and energy. Addition of methanol stimulated methane production only in the case of microbial communities from upper silts. Ability of the communities to produce methane correlated reliably with the concentrations of the NO
3–
, SO
4
2−
, Cl
–
, and CH
3
COO
–
ions in the pore water of the relevant sediments. Cultivation of communities from the mud volcano sediments resulted in development of methanogenic archaea of the family
Methanocellaсеае
in the media supplemented with H
2
: CO
2
and H
2
+ CH
3
COONa, while methanogenic archaea in the communities cultivated without additional substrates belonged to the genera
Methanoregula
,
Methanobacterium
, and
Methanosaeta
.