Agrobacterium mediated genetic transformation and plant regeneration via organogenesis and somatic embryogenesis from cotyledon leaves in eggplant (Solanum melongena L. cv. 'Kecskeméti lila')

• Published in: Plant cell reports Vol. 15; no. 1-2; pp. 82 - 86
• Main Authors: Fári, M, Nagy, I, Csányi, M, Mitykó, J, Andrásfalvy, A
• Format: Journal Article
• Language: English
• Published: Germany 01-01-1995
• Subjects: Agrobacterium; Solanum melongena
• ISSN: 0721-7714; 1432-203X
• DOI: 10.1007/BF01690259

Novel and efficient protocols for plant regeneration and genetic transformation from longitudinally-halved cotyledons ofin vitro raised seedlings in eggplant (Solanum melongena L.) are described. After co-cultivation withAgrobacterium vectors harboring neomycin phosphotransferase (nptll) as selectable marker, transgenic plantlets were regenerated on selective media containing 100 mg/l kanamycin. Transformants were recovered from embryogenic calli induced by 4 mg/lα-naphthaleneacetic acid (NAA), and from organogenic calli induced by the addition of 2 mg/l zeatin plus 0.01 mg/l NAA. Nineteen independent transgenic lines were grown to maturity. The structural integrity, expression and sexual transmission of the introduced genes for neomycin phosphotransferase and ß-glucuronidase (gus) were investigated.