Mass Spectrometric Analysis of Acidic Exo-Oligosaccharides of Root Nodule Bacterium Rhizobium leguminosarum bv. viciae VF39

• Published in: Microbiology (New York) Vol. 89; no. 5; pp. 520 - 531
• Main Authors: Kanapina, A. S., Marchenkov, V. V., Surin, A. K., Ivashina, T. V.
• Format: Journal Article
• Language: English
• Published: Moscow Pleiades Publishing 01-09-2020; Springer Nature B.V
• Subjects: Biomedical and Life Sciences; Exopolysaccharides; Experimental Articles; Life Sciences; Mass spectroscopy; Medical Microbiology; Microbiology; Monomers; Nitrogen fixation; Oligosaccharides; Rhizobium leguminosarum; Trimers; mass spectrometry; exo-oligosaccharide; structure; modifications
• ISSN: 0026-2617; 1608-3237
• DOI: 10.1134/S0026261720050112

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The structures of exo-oligosaccharides produced by a symbiotic nitrogen-fixing bacterium
Rhizobium leguminosarum
bv.
viciae
VF39 were determined using the methods of mass spectrometry and HCD fragmentation of ions. Exo-oligosaccharides were shown to be monomers, dimers and trimers of an octasaccharide repeating unit of the high-molecular-weight acidic exopolysaccharide. The major fraction was represented by octasaccharide monomers with two glucuronic acid residues at the reducing end. Heptasaccharides corresponding to an octasaccharide unit without GlcA-
c
or Glc-
b
residues in the main chain were also found in the culture liquid. Heterogeneity of exo-oligosaccharides according to the content of
O
-acetyl and hydroxybutanoyl substituents was revealed.