Synergistic effect of chitosan and β-carotene in inhibiting MNU-induced retinitis pigmentosa

• Published in: International journal of biological macromolecules Vol. 268; no. Pt 2; p. 131671
• Main Authors: Shi, Enjuan, Wang, Xiaoqin, Jing, Huili, Xu, Yayuan, Feng, Lei, He, Fatao, Li, Dajing, Dai, Zhuqing
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01-05-2024
• Subjects: Chitosan; Gut microbiota; Retinitis pigmentosa; β-Carotene; Gut microbiota; Chitosan; Retinitis pigmentosa; β-Carotene
• ISSN: 0141-8130; 1879-0003
• DOI: 10.1016/j.ijbiomac.2024.131671

In this study, N-Methyl-N-nitrosourea (MNU) was intraperitoneally injected to construct a mouse retinitis pigmentosa (RP) model to evaluate the protective effect of chitosan and β-carotene on RP. The results demonstrated that chitosan synergized with β-carotene significantly reduced retinal histopathological structural damage in RP mice. The co-treatment group of β-carotene and chitosan restored the retinal thickness and outer nuclear layer thickness better than the group treated with the two alone, and the thickness reached the normal level. The content of β-carotene and retinoids in the liver of chitosan and β-carotene co-treated group increased by 46.75 % and 20.69 %, respectively, compared to the β-carotene group. Chitosan and β-carotene supplement suppressed the expressions of Bax, Calpain2, Caspase3, NF-κB, TNF-α, IL-6, and IL-1β, and promoted the up-regulation of Bcl2. Chitosan and β-carotene interventions remarkably contributed to the content of SCFAs and enhanced the abundance of Ruminococcaceae, Rikenellaceae, Odoribacteraceae and Helicobacteraceae. Correlation analysis demonstrated a strong association between gut microbiota and improvement in retinitis pigmentosa. This study will provide a reference for the study of the gut-eye axis.
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•Chitosan synergized with β-carotene inhibits retinal tissue damage.•Chitosan synergized with β-carotene to better inhibit eye inflammation.•Chitosan synergized with β-carotene to better regulate the gut microbiota structure.•There is a strong link between gut microbiota and retinitis pigmentosa.