Establishment and validation of a 2D primary gill cell culture of the sevenband grouper (Hyporthodus septemfasciatus)

A 2D primary gill cell culture system of the sevenband grouper (Hyporthodus septemfasciatus) was established to validate the pathogenesis of nervous necrosis virus (NNV) as observed in previous studies. This system, developed using the double-seeded insert (DSI) technique, yielded confluent cell lay...

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Bibliographic Details
Published in:	Journal of virological methods Vol. 327; p. 114922
Main Authors:	Rajendran, Rahul, Krishnan, Rahul, Oh, Myung-Joo
Format:	Journal Article
Language:	English
Published:	Netherlands Elsevier B.V 01-06-2024
Subjects:	2D primary cell culture Gill epithelium NNV Sevenband grouper Sevenband grouper 2D primary cell culture Gill epithelium NNV
Online Access:	Get full text
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Summary:	A 2D primary gill cell culture system of the sevenband grouper (Hyporthodus septemfasciatus) was established to validate the pathogenesis of nervous necrosis virus (NNV) as observed in previous studies. This system, developed using the double-seeded insert (DSI) technique, yielded confluent cell layers. Upon challenge with NNV in a setup containing both autoclaved salt water and L15 media in the apical compartment, viral replication akin to that anticipated based on previous studies was observed. Consequently, we advocate for the utilization of primary gill cell culture as a viable alternative to conventional methodologies for investigating host pathogen interactions.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 ObjectType-Undefined-3
ISSN:	0166-0934 1879-0984
DOI:	10.1016/j.jviromet.2024.114922