Agrobacterium-mediated transformation of peanut (Arachis hypogaea L.) embryo axes and the development of transgenic plants

• Published in: Plant cell reports Vol. 14; no. 11; pp. 699 - 703
• Main Authors: MCKENTLY, A. H, MOORE, G. A, DOOSTDAR, H, NIEDZ, R. P
• Format: Journal Article
• Language: English
• Published: Berlin Springer 01-08-1995
• Subjects: Biological and medical sciences; Biotechnology; Fundamental and applied biological sciences. Psychology; Genetic engineering; Genetic technics; Methods. Procedures. Technologies; Transgenic animals and transgenic plants; Transgenic plants; Embryo; Vegetals; β-Glucuronidase; Seeds; Genetic transformation; Enzyme; Transferases; Agrobacterium tumefaciens; Arachis hypogaea; Gene expression; Oil plant(vegetal); Kanamycin kinase; Mixed cell culture; Reporter gene; Leguminosae; Dicotyledones; Glycosidases; Angiospermae; Bacteria; Hydrolases; Spermatophyta; Transgenic plant; Rhizobiaceae; O-Glycosidases
• ISSN: 0721-7714; 1432-203X
• DOI: 10.1007/BF00232650

Transgenic peanut (Arachis hypogaea L.) plants have been produced using an Agrobacterium-mediated transformation system. Zygotic embryo axes from mature seed were cocultured with Agrobacterium tumefaciens strain EHA101 harboring a binary vector that contained the genes for the scorable marker B-glucuronidase (GUS) and the selectable marker neomycin phosphotransferase II. Nine percent of the germinated seedlings were GUS+. Polymerase chain reaction analysis confirmed that GUS+ shoots and T1 progeny contained T-DNA. Molecular characterization of one primary transformant and its T1 and T2 progeny plants established that T-DNA was integrated into the host genome.