Countercurrent Chromatographic Purification of Polysaccharides from Achyranthes bidentata with an Aqueous Two-Phase System Using a Cross-Axis Coil Planet Centrifuge

Polysaccharides were purified from a high molecular weight complex of Achyranthes bidentata Bl. (Fam. Amaranthaceae) root by high speed countercurrent chromatography (HSCCC) using a cross-axis coil planet centrifuge (X-axis CPC). The purification was performed with an aqueous polymer two-phase syste...

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Published in:	Journal of liquid chromatography & related technologies Vol. 26; no. 12; pp. 1895 - 1903
Main Authors:	Chao, Zhimao, Shibusawa, Yoichi, Shindo, Heisaburo, Ito, Yoichiro
Format:	Journal Article
Language:	English
Published:	Colchester Taylor & Francis Group 01-01-2003 Taylor & Francis
Subjects:	Biological and medical sciences Countercurrent chromatography Cross-axis coil planet centrifuge General pharmacology Medical sciences Partition coefficient Pharmacognosy. Homeopathy. Health food Pharmacology. Drug treatments Polysaccharides Asia China Countercurrent chromatography Purification Root Preparative chromatography Amaranthaceae Medicinal plant Separation method Folk medicine Dicotyledones Angiospermae Plant origin Isolation Spermatophyta Polysaccharide
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Abstract	Polysaccharides were purified from a high molecular weight complex of Achyranthes bidentata Bl. (Fam. Amaranthaceae) root by high speed countercurrent chromatography (HSCCC) using a cross-axis coil planet centrifuge (X-axis CPC). The purification was performed with an aqueous polymer two-phase system composed of 12.5% (w/w) polyethylene glycol (PEG) 1000, 8% (w/w) potassium dihydrogen phosphate, and 8% (w/w) dipotassium hydrogen phosphate buffer at pH 6.8. The preparative separation of the crude sample (2 g) was successfully performed, yielding 1.1 g of polysaccharides at high purity.
AbstractList	Polysaccharides were purified from a high molecular weight complex of Achyranthes bidentata Bl. (Fam. Amaranthaceae) root by high speed countercurrent chromatography (HSCCC) using a cross-axis coil planet centrifuge (X-axis CPC). The purification was performed with an aqueous polymer two-phase system composed of 12.5% (w/w) polyethylene glycol (PEG) 1000, 8% (w/w) potassium dihydrogen phosphate, and 8% (w/w) dipotassium hydrogen phosphate buffer at pH 6.8. The preparative separation of the crude sample (2 g) was successfully performed, yielding 1.1 g of polysaccharides at high purity.
Author	Shindo, Heisaburo Ito, Yoichiro Chao, Zhimao Shibusawa, Yoichi
Author_xml	– sequence: 1 givenname: Zhimao surname: Chao fullname: Chao, Zhimao email: chaozhimao@sina.com organization: Institute of Chinese Materia Medica , China Academy of Traditional Chinese Medicine – sequence: 2 givenname: Yoichi surname: Shibusawa fullname: Shibusawa, Yoichi organization: Department of Analytical Chemistry, School of Pharmacy , Tokyo University of Pharmacy and Life Science – sequence: 3 givenname: Heisaburo surname: Shindo fullname: Shindo, Heisaburo organization: Department of Analytical Chemistry, School of Pharmacy , Tokyo University of Pharmacy and Life Science – sequence: 4 givenname: Yoichiro surname: Ito fullname: Ito, Yoichiro organization: Laboratory of Biophysical Chemistry, National Heart, Lung, and Blood Institute , National Institutes of Health
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CitedBy_id	crossref_primary_10_1002_jssc_201700340 crossref_primary_10_1016_j_chroma_2006_11_072 crossref_primary_10_1016_j_seppur_2013_04_004 crossref_primary_10_1002_jssc_201501199 crossref_primary_10_1016_j_biteb_2021_100758 crossref_primary_10_1016_j_chroma_2012_08_099 crossref_primary_10_1002_pca_746 crossref_primary_10_1071_CH17004 crossref_primary_10_1016_j_ijbiomac_2023_128282 crossref_primary_10_1002_jssc_201201079 crossref_primary_10_1016_j_chroma_2008_10_071 crossref_primary_10_1002_jssc_201500907 crossref_primary_10_1016_j_jchromb_2015_12_051
Cites_doi	10.1016/S0021-9673(01)84486-1 10.1080/10826079208016348 10.1081/JLC-100104442
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Keywords	Countercurrent chromatography Purification Root Preparative chromatography Amaranthaceae Medicinal plant Separation method Folk medicine Dicotyledones Angiospermae Plant origin Isolation Spermatophyta Polysaccharide
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References	CIT17580005 CIT17580007 Chao Z. (CIT17580006) 1999; 34 Xiang D. B. (CIT17580002) 1993; 14 CIT17580004 Zhang R. L. (CIT17580001) 1981; 3 Xiang D. B. (CIT17580003) 1993; 14
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Snippet	Polysaccharides were purified from a high molecular weight complex of Achyranthes bidentata Bl. (Fam. Amaranthaceae) root by high speed countercurrent...
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SubjectTerms	Biological and medical sciences Countercurrent chromatography Cross-axis coil planet centrifuge General pharmacology Medical sciences Partition coefficient Pharmacognosy. Homeopathy. Health food Pharmacology. Drug treatments Polysaccharides
Title	Countercurrent Chromatographic Purification of Polysaccharides from Achyranthes bidentata with an Aqueous Two-Phase System Using a Cross-Axis Coil Planet Centrifuge
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