Candidiasis and the impact of flow cytometry on antifungal drug discovery

• Published in: Expert opinion on drug discovery Vol. 12; no. 11; p. 1127
• Main Authors: Ku, Tsun Sheng N, Bernardo, Stella, Walraven, Carla J, Lee, Samuel A
• Format: Journal Article
• Language: English
• Published: England 02-11-2017
• Subjects: Antifungal Agents - pharmacology; Candidiasis - drug therapy; Candidiasis - microbiology; Drug Design; Drug Discovery - methods; Drug Resistance, Fungal; Flow Cytometry - methods; High-Throughput Screening Assays - methods; Humans; flow cytometry; Antifungal; drug repurposing; drug discover; Candida; biofilm
• ISSN: 1746-045X
• DOI: 10.1080/17460441.2017.1377179

Invasive candidiasis continues to be associated with significant morbidity and mortality as well as substantial health care costs nationally and globally. One of the contributing factors is the development of resistance to antifungal agents that are already in clinical use. Moreover, there are known treatment limitations with all of the available antifungal agents. Since traditional techniques in novel drug discovery are time consuming, high-throughput screening using flow cytometry presents as a potential tool to identify new antifungal agents that would be useful in the management of these patients. Areas covered: In this review, the authors discuss the use of automated high-throughput screening assays based upon flow cytometry to identify potential antifungals from a library comprised of a large number of bioactive compounds. They also review studies that employed the use of this research methodology that has identified compounds with antifungal activity. Expert opinion: High-throughput screening using flow cytometry has substantially decreased the processing time necessary for screening thousands of compounds, and has helped enhance our understanding of fungal pathogenesis. Indeed, the authors see this technology as a powerful tool to help scientists identify new antifungal agents that can be added to the clinician's arsenal in their fight against invasive candidiasis.