Gradients in Susceptibility and Resistance Mechanisms of Buddleia L. Taxa to the Two-spotted Spider Mite (Tetranychus urticae Koch)

• Published in: Journal of the American Society for Horticultural Science Vol. 124; no. 2; pp. 114 - 121
• Main Authors: Gillman, Jeffrey H, Dirr, Michael A, Braman, S. Kristine
• Format: Journal Article
• Language: English
• Published: 01-03-1999
• ISSN: 0003-1062; 2327-9788
• DOI: 10.21273/JASHS.124.2.114

Buddleia
taxa were assessed for two-spotted spider mite (
Tetranychus urticae
Koch) resistance using a leaf disk bioassay, a novel shell vial bioassay and a field trial. Leaf pubescence and chemistry were examined for their role in two-spotted spider mite resistance. Results from bioassays and field sampling identified highly resistant taxa including
B. fallowiana
Balif. `Alba' and
B
.
davidii
×
B. fallowiana
Franch. `Cornwall Blue' as well as susceptible taxa including
B. davidii
Franch. `African Queen' and
B. lindleyana
Fort. ex Lindl. `Gloster'. The shell vial bioassay was an accurate predictor of field resistance to spider mite. Leaf pubescence was quantified by calculating the collective length of trichome branches per square millimeter of leaf surface area [effective branch length (EBL)]. EBL values ranged from 39 to 162 mm·mm
-2
of leaf surface area among
Buddleia
taxa. Resistance was positively correlated with increased pubescence. Removal of pubescence by peeling resulted in increased oviposition of two-spotted spider mites. Exposing female two-spotted spider mites to a methylene chloride extract of
B
.
davidii
×
B. fallowiana
`Cornwall Blue' using a modified shell vial bioassay resulted in reduced oviposition and a methylene chloride extract of
B. davidii
`African Queen' resulted in no difference in oviposition when compared with a control. While pubescence is the best indicator of resistance to the two-spotted spider mite in
Buddleia
taxa, it is possible that defensive compounds are involved.