Genetic fingerprinting of pigeonpea [Cajanus cajan(L.) Millsp.] and its wild relatives using RAPD markers

• Published in: Theoretical and applied genetics Vol. 91; no. 6-7; pp. 893 - 898
• Main Authors: RATNAPARKHE, M. B, GUPTA, V. S, VEN MURTHY, M. R, RANJEKAR, P. K
• Format: Journal Article
• Language: English
• Published: Heidelberg Springer 01-11-1995; Berlin
• Subjects: Biological and medical sciences; Classical genetics, quantitative genetics, hybrids; Fundamental and applied biological sciences. Psychology; Genetics of eukaryotes. Biological and molecular evolution; Pteridophyta, spermatophyta; Vegetals; Genetic variability; Genetic marker; Biological evolution; Cajanus cajan; Genetic distance; Phylogeny; Grain legume; Leguminosae; Random amplified polymorphic DNA; Dicotyledones; Angiospermae; Spermatophyta; Cultivar; Polymorphism
• ISSN: 0040-5752; 1432-2242
• DOI: 10.1007/BF00223897

Randomly amplified polymorphic DNA (RAPD) markers were used for the identification of pigeonpea [Cajanus cajan (L.) Millsp.] cultivars and their related wild species. The use of single primers of arbitrary nucleotide sequence resulted in the selective amplification of DNA fragments that were unique to individual accessions. The level of polymorphism among the wild species was extremely high, while little polymorphism was detected within Cajanus cajan accessions. All of the cultivars and wild species under study could be easily distinguished with the help of different primers, thereby indicating the immense potential of RAPD in the genetic fingerprinting of pigeonpea. On the basis of our data the genetic relationship between pigeonpea cultivars and its wild species could be established.