Vaginal Mucosal Melanoma Cell Activation in Response to Photon or Carbon Ion Irradiation

• Published in: International journal of particle therapy Vol. 14; p. 100630
• Main Authors: Charalampopoulou, Alexandra, Barcellini, Amelia, Bistika, Margarita, Ivaldi, Giovanni Battista, Lillo, Sara, Magro, Giuseppe, Orlandi, Ester, Pullia, Marco Giuseppe, Ronchi, Sara, De Fatis, Paola Tabarelli, Facoetti, Angelica
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 01-12-2024; Particle Therapy Cooperative Group (PTCOG)
• Subjects: Carbon ions; Melanin; Migration; Mucosal malignant melanoma; Original; Radiobiology; Radiobiology; Migration; Carbon ions; Mucosal malignant melanoma; Melanin
• ISSN: 2331-5180; 2331-5180
• DOI: 10.1016/j.ijpt.2024.100630

Primary gynecological melanomas are uncommon with lower survival rates compared to cutaneous melanomas. Although melanocytes have been identified in a variety of mucosal membranes, little is known about their interactions or roles inside the mucosa layer. Melanin is a common pigment in nature and is endowed with several peculiar chemical, paramagnetic, and semiconductive characteristics. One of its latest explored functions is its interaction with ionizing radiation as a protective mechanism as well as its implication in the metastatic cascade of tumor cells.
In this work, we analyzed in vitro the effects of different doses of photon and carbon ion irradiation on dendrite formation, pigmentation, migration, and invasion abilities of human mucosal melanoma cells of the vagina. We evaluated the morphology and melanin production of HMV-II cells exposed to photon and carbon ion beams with single doses between 0.5 and 10 Gy.
Our results showed that irradiation induces dendrite formation or elongation and pigmentation in HMV-II cells in a dose-type-dependent and radiation-type-dependent way but also a decrease in cell motility.
The present study describes for the first time an induction of dendritic formation, melanin production, and alterations in migration and invasion abilities by low-linear energy transfer and high-linear energy transfer radiation in human mucosal melanoma cells, suggesting a radioprotective response to further possible exposures increasing the radioresistance of these cells.