Route of jejunal mucosa absorption of trypsin demonstrated by immunofluorescence

• Published in: Journal of molecular histology Vol. 34; no. 11-12; p. 525
• Main Authors: Borges, Elizabeth L, de Fátima Leite, Maria, Barbosa, Alfredo J A, Alves, José B
• Format: Journal Article
• Language: English
• Published: Netherlands Springer Nature B.V 01-07-2003
• Subjects: Animals; Fluorescent Antibody Technique, Indirect; Immunoenzyme Techniques; Intestinal Absorption - physiology; Intestinal Mucosa - metabolism; Jejunum - metabolism; Male; Perfusion; Rats; Rats, Wistar; Rodents; Trypsin - metabolism
• ISSN: 0018-2214; 1567-2379; 1567-2387
• DOI: 10.1023/A:1026020301650

Previous studies have demonstrated the absorption of porcine trypsin in isolated jejunal loops from male Wistar rats by open-loop perfusion. The possible routes of absorption were examined in the study reported here. Trypsin (0.5 mg/ml) was dissolved in tyrode solution and perfused at a rate of 0.5 ml/min, at 37 degrees C, for 40 min. Using immunoperoxidase and immunofluorescence techniques, strong reactivity towards anti-TLCK-trypsin antibody was demonstrated through out the enterocyte cytosol. The present data indicate that trypsin was absorbed by enterocytes, probably through a transcellular route.