Elucidation of clearance mechanism of TP0463518, a novel hypoxia-inducible factor prolyl hydroxylase inhibitor: does a species difference in excretion routes exist between humans and animals?

• Published in: Xenobiotica Vol. 52; no. 7; pp. 729 - 741
• Main Authors: Takano, Hiroki, Mizuno-Yasuhira, Akiko, Yamaguchi, Jun-ichi, Endo, Hiromi
• Format: Journal Article
• Language: English
• Published: England Taylor & Francis 03-07-2022
• Subjects: clearance mechanism; hepatic uptake; HIF-PH inhibitor; OAT; OATP; species difference; TP0463518; urinary excretion; clearance mechanism; species difference; OAT; urinary excretion; TP0463518; hepatic uptake; OATP; HIF-PH inhibitor
• ISSN: 0049-8254; 1366-5928
• DOI: 10.1080/00498254.2022.2147038

1. TP0463518, a novel hypoxia-inducible factor prolyl hydroxylase inhibitor, is reportedly excreted predominantly through urinary excretion in an unchanged form in humans, with partial biliary excretion also possible. However, the clearance mechanisms remain unclear. The aim of this study was to investigate the clearance mechanisms in humans and to assess species differences in the excretion routes.
2. TP0463518 was not metabolised in rat, dog, or human hepatocytes. TP0463518 is a substrate for human BCRP, OATP1B1, OATP1B3, and OAT3, suggesting that renal uptake by OAT3 is probably the predominant clearance route, with hepatic uptake by OATP1B1 and OATP1B3 contributing partially to clearance in humans.
3. A species difference in excretion routes was observed. The unchanged urinary excretion rates in humans, male rats, female rats, dogs, and monkeys were 80.7%, 0.1%, 40.9%, 15.2%, and 72.6%, respectively. Urinary excretion was predominant in humans and monkeys, while only biliary excretion was observed in male rats. Uptake studies using hepatocytes showed that the hepatic uptake clearance in rats was 13.6-fold higher than that in humans. Therefore, not only reabsorption via renal tubules, but also hepatic uptake seems to be involved in the species differences in excretion routes between rats and humans.