Highly multiplexed design of an allosteric transcription factor to sense new ligands
Allosteric transcription factors (aTF) regulate gene expression through conformational changes induced by small molecule binding. Although widely used as biosensors, aTFs have proven challenging to design for detecting new molecules because mutation of ligand-binding residues often disrupts alloster...
Saved in:
| Published in: | Nature communications Vol. 15; no. 1; pp. 10001 - 18 |
|---|---|
| Main Authors: | , , , , , , , , , , , , |
| Format: | Journal Article |
| Language: | English |
| Published: |
London
Nature Publishing Group UK
19-11-2024
Nature Publishing Group Nature Portfolio |
| Subjects: | |
| Online Access: | Get full text |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Abstract | Allosteric transcription factors (aTF) regulate gene expression through conformational changes induced by small molecule binding. Although widely used as biosensors, aTFs have proven challenging to design for detecting new molecules because mutation of ligand-binding residues often disrupts allostery. Here, we develop Sensor-seq, a high-throughput platform to design and identify aTF biosensors that bind to non-native ligands. We screen a library of 17,737 variants of the aTF TtgR, a regulator of a multidrug exporter, against six non-native ligands of diverse chemical structures – four derivatives of the cancer therapeutic tamoxifen, the antimalarial drug quinine, and the opiate analog naltrexone – as well as two native flavonoid ligands, naringenin and phloretin. Sensor-seq identifies biosensors for each of these ligands with high dynamic range and diverse specificity profiles. The structure of a naltrexone-bound design shows shape-complementary methionine-aromatic interactions driving ligand specificity. To demonstrate practical utility, we develop cell-free detection systems for naltrexone and quinine. Sensor-seq enables rapid and scalable design of new biosensors, overcoming constraints of natural biosensors.
The use of allosteric transcription factors (aTFs) as biosensors has been constrained by their limited natural ligand repertoire. Here, the authors report a method to screen large libraries of aTF variants to develop biosensors with altered specificities to non-native ligands. |
|---|---|
| AbstractList | Allosteric transcription factors (aTF) regulate gene expression through conformational changes induced by small molecule binding. Although widely used as biosensors, aTFs have proven challenging to design for detecting new molecules because mutation of ligand-binding residues often disrupts allostery. Here, we develop Sensor-seq, a high-throughput platform to design and identify aTF biosensors that bind to non-native ligands. We screen a library of 17,737 variants of the aTF TtgR, a regulator of a multidrug exporter, against six non-native ligands of diverse chemical structures – four derivatives of the cancer therapeutic tamoxifen, the antimalarial drug quinine, and the opiate analog naltrexone – as well as two native flavonoid ligands, naringenin and phloretin. Sensor-seq identifies biosensors for each of these ligands with high dynamic range and diverse specificity profiles. The structure of a naltrexone-bound design shows shape-complementary methionine-aromatic interactions driving ligand specificity. To demonstrate practical utility, we develop cell-free detection systems for naltrexone and quinine. Sensor-seq enables rapid and scalable design of new biosensors, overcoming constraints of natural biosensors.
The use of allosteric transcription factors (aTFs) as biosensors has been constrained by their limited natural ligand repertoire. Here, the authors report a method to screen large libraries of aTF variants to develop biosensors with altered specificities to non-native ligands. Allosteric transcription factors (aTF) regulate gene expression through conformational changes induced by small molecule binding. Although widely used as biosensors, aTFs have proven challenging to design for detecting new molecules because mutation of ligand-binding residues often disrupts allostery. Here, we develop Sensor-seq, a high-throughput platform to design and identify aTF biosensors that bind to non-native ligands. We screen a library of 17,737 variants of the aTF TtgR, a regulator of a multidrug exporter, against six non-native ligands of diverse chemical structures – four derivatives of the cancer therapeutic tamoxifen, the antimalarial drug quinine, and the opiate analog naltrexone – as well as two native flavonoid ligands, naringenin and phloretin. Sensor-seq identifies biosensors for each of these ligands with high dynamic range and diverse specificity profiles. The structure of a naltrexone-bound design shows shape-complementary methionine-aromatic interactions driving ligand specificity. To demonstrate practical utility, we develop cell-free detection systems for naltrexone and quinine. Sensor-seq enables rapid and scalable design of new biosensors, overcoming constraints of natural biosensors.The use of allosteric transcription factors (aTFs) as biosensors has been constrained by their limited natural ligand repertoire. Here, the authors report a method to screen large libraries of aTF variants to develop biosensors with altered specificities to non-native ligands. Abstract Allosteric transcription factors (aTF) regulate gene expression through conformational changes induced by small molecule binding. Although widely used as biosensors, aTFs have proven challenging to design for detecting new molecules because mutation of ligand-binding residues often disrupts allostery. Here, we develop Sensor-seq, a high-throughput platform to design and identify aTF biosensors that bind to non-native ligands. We screen a library of 17,737 variants of the aTF TtgR, a regulator of a multidrug exporter, against six non-native ligands of diverse chemical structures – four derivatives of the cancer therapeutic tamoxifen, the antimalarial drug quinine, and the opiate analog naltrexone – as well as two native flavonoid ligands, naringenin and phloretin. Sensor-seq identifies biosensors for each of these ligands with high dynamic range and diverse specificity profiles. The structure of a naltrexone-bound design shows shape-complementary methionine-aromatic interactions driving ligand specificity. To demonstrate practical utility, we develop cell-free detection systems for naltrexone and quinine. Sensor-seq enables rapid and scalable design of new biosensors, overcoming constraints of natural biosensors. Allosteric transcription factors (aTF) regulate gene expression through conformational changes induced by small molecule binding. Although widely used as biosensors, aTFs have proven challenging to design for detecting new molecules because mutation of ligand-binding residues often disrupts allostery. Here, we develop Sensor-seq, a high-throughput platform to design and identify aTF biosensors that bind to non-native ligands. We screen a library of 17,737 variants of the aTF TtgR, a regulator of a multidrug exporter, against six non-native ligands of diverse chemical structures - four derivatives of the cancer therapeutic tamoxifen, the antimalarial drug quinine, and the opiate analog naltrexone - as well as two native flavonoid ligands, naringenin and phloretin. Sensor-seq identifies biosensors for each of these ligands with high dynamic range and diverse specificity profiles. The structure of a naltrexone-bound design shows shape-complementary methionine-aromatic interactions driving ligand specificity. To demonstrate practical utility, we develop cell-free detection systems for naltrexone and quinine. Sensor-seq enables rapid and scalable design of new biosensors, overcoming constraints of natural biosensors.Allosteric transcription factors (aTF) regulate gene expression through conformational changes induced by small molecule binding. Although widely used as biosensors, aTFs have proven challenging to design for detecting new molecules because mutation of ligand-binding residues often disrupts allostery. Here, we develop Sensor-seq, a high-throughput platform to design and identify aTF biosensors that bind to non-native ligands. We screen a library of 17,737 variants of the aTF TtgR, a regulator of a multidrug exporter, against six non-native ligands of diverse chemical structures - four derivatives of the cancer therapeutic tamoxifen, the antimalarial drug quinine, and the opiate analog naltrexone - as well as two native flavonoid ligands, naringenin and phloretin. Sensor-seq identifies biosensors for each of these ligands with high dynamic range and diverse specificity profiles. The structure of a naltrexone-bound design shows shape-complementary methionine-aromatic interactions driving ligand specificity. To demonstrate practical utility, we develop cell-free detection systems for naltrexone and quinine. Sensor-seq enables rapid and scalable design of new biosensors, overcoming constraints of natural biosensors. |
| ArticleNumber | 10001 |
| Author | Fox, Brian G. Nishikawa, Kyle K. Chávez, Jorge L. Acheson, Justin F. Harbaugh, Svetlana V. Lee, Daniel H. Chen, Jackie Novy, Nathan Lodewyk, Ella C. Raman, Srivatsan Frenkel, Max Huss, Phil Sieren, Hailey R. |
| Author_xml | – sequence: 1 givenname: Kyle K. surname: Nishikawa fullname: Nishikawa, Kyle K. organization: Department of Biochemistry, University of Wisconsin-Madison – sequence: 2 givenname: Jackie surname: Chen fullname: Chen, Jackie organization: Department of Biochemistry, University of Wisconsin-Madison – sequence: 3 givenname: Justin F. orcidid: 0000-0001-5051-9984 surname: Acheson fullname: Acheson, Justin F. organization: Department of Biochemistry, University of Wisconsin-Madison – sequence: 4 givenname: Svetlana V. surname: Harbaugh fullname: Harbaugh, Svetlana V. organization: 711th Human Performance Wing, Air Force Research Laboratory – sequence: 5 givenname: Phil surname: Huss fullname: Huss, Phil organization: Department of Biochemistry, University of Wisconsin-Madison – sequence: 6 givenname: Max surname: Frenkel fullname: Frenkel, Max organization: Department of Biochemistry, University of Wisconsin-Madison – sequence: 7 givenname: Nathan surname: Novy fullname: Novy, Nathan organization: Department of Biochemistry, University of Wisconsin-Madison – sequence: 8 givenname: Hailey R. surname: Sieren fullname: Sieren, Hailey R. organization: Department of Biochemistry, University of Wisconsin-Madison, Dane County Youth Apprenticeship Program, State of Wisconsin Department of Workforce Development – sequence: 9 givenname: Ella C. surname: Lodewyk fullname: Lodewyk, Ella C. organization: Department of Biochemistry, University of Wisconsin-Madison, Dane County Youth Apprenticeship Program, State of Wisconsin Department of Workforce Development – sequence: 10 givenname: Daniel H. surname: Lee fullname: Lee, Daniel H. organization: Department of Biochemistry, University of Wisconsin-Madison, Dane County Youth Apprenticeship Program, State of Wisconsin Department of Workforce Development – sequence: 11 givenname: Jorge L. surname: Chávez fullname: Chávez, Jorge L. organization: 711th Human Performance Wing, Air Force Research Laboratory – sequence: 12 givenname: Brian G. surname: Fox fullname: Fox, Brian G. organization: Department of Biochemistry, University of Wisconsin-Madison, Great Lakes Bioenergy Research Center, University of Wisconsin-Madison – sequence: 13 givenname: Srivatsan orcidid: 0000-0003-2461-1589 surname: Raman fullname: Raman, Srivatsan email: sraman4@wisc.edu organization: Department of Biochemistry, University of Wisconsin-Madison, Great Lakes Bioenergy Research Center, University of Wisconsin-Madison, Department of Bacteriology, University of Wisconsin-Madison, Department of Chemical and Biological Engineering, University of Wisconsin-Madison |
| BookMark | eNp9kc1u1TAQhSNUJErpC7CyxIZNwL9xskKoAlqpEpuytsb2JPWVr32xE6Bv3_SmAsoCb2yNz_k0M-dlc5JywqZ5zeg7RkX_vkomO91SLlsleUfb_llzyqlkLdNcnPz1ftGc17qj6xED66U8bW4uw3Qb78h-iXM4RPyFnnisYUokjwQSgRhznbEER-YCqboSDnPIiYzg5lzInEnFVJEk_ElimCD5-qp5PkKseP54nzXfPn-6ubhsr79-ubr4eN06wWTf9lri0HmLHe_ZYFWngCsBohsFtdBJIQG8760VahwoY9R6oThYSSlqzqk4a642rs-wM4cS9lDuTIZgjoVcJgNlDi6iQaUddAL0ILj0rLdq0Aos5VpIZ5ldWR821mGxe_QO0zpufAJ9-pPCrZnyD8OY0poytRLePhJK_r5gnc0-VIcxQsK8VCOYoL2gg3ho_M0_0l1eSlp3dVSpTg-crSq-qVzJtRYcf3fDqHlI3mzJmzV5c0ze9KtJbKa6itOE5Q_6P657jFaySg |
| Cites_doi | 10.1021/acs.jpcb.8b05795 10.1111/j.1365-2958.2007.05832.x 10.1038/nsb881 10.1093/nar/gks806 10.1038/ng1482 10.1073/pnas.1406401111 10.1107/S0907444904019158 10.1038/s41592-019-0686-2 10.7554/eLife.54895 10.1038/s41594-021-00569-7 10.1016/j.copbio.2021.01.008 10.1016/j.molcel.2018.08.033 10.1146/annurev-biochem-062608-095938 10.1023/B:BREA.0000025406.31193.e8 10.1021/sb300091d 10.1107/S0907444911007773 10.1038/nmeth.3696 10.3390/antibiotics11040520 10.1016/j.ymben.2012.02.002 10.1038/s41592-018-0138-4 10.1073/pnas.2104878118 10.1016/j.abb.2019.07.018 10.1016/j.tibs.2023.05.009 10.1074/jbc.M112.374504 10.1038/s41467-021-25826-7 10.1016/j.ymeth.2013.06.027 10.1021/acssynbio.7b00114 10.1016/j.copbio.2016.03.005 10.1073/pnas.2002613117 10.1002/pro.3774 10.1107/S2059798319011471 10.1128/AEM.66.1.163-169.2000 10.1002/jcp.28720 10.1093/nar/gkaa786 10.1186/s12859-018-2579-2 10.1186/s12864-016-2533-5 10.1093/nar/27.1.368 10.1073/pnas.1409523111 10.1016/B978-0-12-381270-4.00019-6 10.1155/2022/3848084 10.21105/joss.00861 10.1021/acssynbio.9b00348 10.5858/arpa.2020-0055-RA 10.1002/anie.201006083 10.1186/1475-2875-10-144 10.1126/science.add2187 10.1073/pnas.1301301110 10.1016/j.jmb.2007.03.062 10.1002/prot.24828 10.1038/nbt.3769 10.1093/nar/gkg037 10.1007/s00253-015-7090-3 10.1016/j.sbi.2022.102334 10.1021/acssynbio.8b00430 10.7554/eLife.79932 10.1038/nbt.2149 10.1128/MMBR.00018-13 10.1021/acssynbio.2c00382 10.1021/acs.jctc.7b00125 10.1007/s00253-019-10179-5 10.1371/journal.pone.0138469 10.3389/fbioe.2020.00098 10.1007/s40263-013-0096-4 10.1007/s10311-016-0582-2 10.1093/bioinformatics/btt593 10.1038/s41587-020-0571-7 10.7554/eLife.07864 10.1093/bioinformatics/bty560 10.1186/1471-2164-14-745 10.1107/S0907444909047337 10.1038/sj.npp.1301440 10.1101/gr.209601.116 10.3389/fbioe.2023.1118702 10.1007/978-3-642-37456-2_14 10.1002/pro.4780 |
| ContentType | Journal Article |
| Copyright | The Author(s) 2024 The Author(s) 2024. This work is published under http://creativecommons.org/licenses/by-nc-nd/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. 2024. The Author(s). The Author(s) 2024 2024 |
| Copyright_xml | – notice: The Author(s) 2024 – notice: The Author(s) 2024. This work is published under http://creativecommons.org/licenses/by-nc-nd/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. – notice: 2024. The Author(s). – notice: The Author(s) 2024 2024 |
| DBID | C6C AAYXX CITATION 3V. 7QL 7QP 7QR 7SN 7SS 7ST 7T5 7T7 7TM 7TO 7X7 7XB 88E 8AO 8FD 8FE 8FG 8FH 8FI 8FJ 8FK ABUWG AFKRA ARAPS AZQEC BBNVY BENPR BGLVJ BHPHI C1K CCPQU DWQXO FR3 FYUFA GHDGH GNUQQ H94 HCIFZ K9. LK8 M0S M1P M7P P5Z P62 P64 PIMPY PQEST PQQKQ PQUKI PRINS RC3 SOI 7X8 5PM DOA |
| DOI | 10.1038/s41467-024-54260-8 |
| DatabaseName | SpringerOpen CrossRef ProQuest Central (Corporate) Bacteriology Abstracts (Microbiology B) Calcium & Calcified Tissue Abstracts Chemoreception Abstracts Ecology Abstracts Entomology Abstracts (Full archive) Environment Abstracts Immunology Abstracts Industrial and Applied Microbiology Abstracts (Microbiology A) Nucleic Acids Abstracts Oncogenes and Growth Factors Abstracts Health & Medical Collection ProQuest Central (purchase pre-March 2016) Medical Database (Alumni Edition) ProQuest Pharma Collection Technology Research Database ProQuest SciTech Collection ProQuest Technology Collection ProQuest Natural Science Collection Hospital Premium Collection Hospital Premium Collection (Alumni Edition) ProQuest Central (Alumni) (purchase pre-March 2016) ProQuest Central (Alumni) ProQuest Central UK/Ireland Advanced Technologies & Aerospace Collection ProQuest Central Essentials Biological Science Collection ProQuest Databases Technology Collection ProQuest Natural Science Collection Environmental Sciences and Pollution Management ProQuest One Community College ProQuest Central Engineering Research Database Health Research Premium Collection Health Research Premium Collection (Alumni) ProQuest Central Student AIDS and Cancer Research Abstracts SciTech Premium Collection ProQuest Health & Medical Complete (Alumni) Biological Sciences Health & Medical Collection (Alumni Edition) PML(ProQuest Medical Library) Biological Science Database ProQuest Advanced Technologies & Aerospace Database ProQuest Advanced Technologies & Aerospace Collection Biotechnology and BioEngineering Abstracts Publicly Available Content Database ProQuest One Academic Eastern Edition (DO NOT USE) ProQuest One Academic ProQuest One Academic UKI Edition ProQuest Central China Genetics Abstracts Environment Abstracts MEDLINE - Academic PubMed Central (Full Participant titles) Directory of Open Access Journals |
| DatabaseTitle | CrossRef Publicly Available Content Database ProQuest Central Student Oncogenes and Growth Factors Abstracts ProQuest Advanced Technologies & Aerospace Collection ProQuest Central Essentials Nucleic Acids Abstracts SciTech Premium Collection ProQuest Central China Environmental Sciences and Pollution Management Health Research Premium Collection Natural Science Collection Biological Science Collection Chemoreception Abstracts Industrial and Applied Microbiology Abstracts (Microbiology A) ProQuest Medical Library (Alumni) Advanced Technologies & Aerospace Collection ProQuest Biological Science Collection ProQuest One Academic Eastern Edition ProQuest Hospital Collection ProQuest Technology Collection Health Research Premium Collection (Alumni) Biological Science Database Ecology Abstracts ProQuest Hospital Collection (Alumni) Biotechnology and BioEngineering Abstracts Entomology Abstracts ProQuest Health & Medical Complete ProQuest One Academic UKI Edition Engineering Research Database ProQuest One Academic Calcium & Calcified Tissue Abstracts Technology Collection Technology Research Database ProQuest Health & Medical Complete (Alumni) ProQuest Central (Alumni Edition) ProQuest One Community College ProQuest Natural Science Collection ProQuest Pharma Collection ProQuest Central Genetics Abstracts Health and Medicine Complete (Alumni Edition) ProQuest Central Korea Bacteriology Abstracts (Microbiology B) AIDS and Cancer Research Abstracts ProQuest SciTech Collection Advanced Technologies & Aerospace Database ProQuest Medical Library Immunology Abstracts Environment Abstracts ProQuest Central (Alumni) MEDLINE - Academic |
| DatabaseTitleList | Publicly Available Content Database MEDLINE - Academic |
| Database_xml | – sequence: 1 dbid: DOA name: Directory of Open Access Journals url: http://www.doaj.org/ sourceTypes: Open Website |
| DeliveryMethod | fulltext_linktorsrc |
| Discipline | Biology |
| EISSN | 2041-1723 |
| EndPage | 18 |
| ExternalDocumentID | oai_doaj_org_article_e57ca63a79324d18b5975ab02734cb1b 10_1038_s41467_024_54260_8 |
| GrantInformation_xml | – fundername: United States Department of Defense | United States Army | U.S. Army Research, Development and Engineering Command | Army Research Office (ARO) grantid: W911NF1710043; W911NF20C0005 funderid: https://doi.org/10.13039/100000183 |
| GroupedDBID | --- 0R~ 39C 3V. 53G 5VS 70F 7X7 88E 8AO 8FE 8FG 8FH 8FI 8FJ AAHBH AAJSJ ABUWG ACGFO ACGFS ACIWK ACMJI ACPRK ACSMW ADBBV ADFRT ADRAZ AENEX AFKRA AFRAH AHMBA AJTQC ALIPV ALMA_UNASSIGNED_HOLDINGS AMTXH AOIJS ARAPS ASPBG AVWKF AZFZN BBNVY BCNDV BENPR BGLVJ BHPHI BPHCQ BVXVI C6C CCPQU DIK EBLON EBS EE. EMOBN F5P FEDTE FYUFA GROUPED_DOAJ HCIFZ HMCUK HVGLF HYE HZ~ KQ8 LGEZI LK8 LOTEE M1P M7P M~E NADUK NAO NXXTH O9- OK1 P2P P62 PIMPY PQQKQ PROAC PSQYO RNS RNT RNTTT RPM SNYQT SV3 TSG UKHRP AAYXX CITATION 7QL 7QP 7QR 7SN 7SS 7ST 7T5 7T7 7TM 7TO 7XB 8FD 8FK AZQEC C1K DWQXO FR3 GNUQQ H94 K9. M48 P64 PQEST PQUKI PRINS RC3 SOI 7X8 5PM |
| ID | FETCH-LOGICAL-c3148-874e96dbe62819b565a253a36f30ba6434aadd8bb35f90110bd352ab400e72203 |
| IEDL.DBID | RPM |
| ISSN | 2041-1723 |
| IngestDate | Mon Nov 25 20:28:58 EST 2024 Thu Nov 21 05:38:07 EST 2024 Tue Nov 26 19:31:55 EST 2024 Wed Nov 20 09:12:50 EST 2024 Wed Nov 27 12:58:17 EST 2024 Thu Nov 21 03:04:52 EST 2024 |
| IsDoiOpenAccess | true |
| IsOpenAccess | true |
| IsPeerReviewed | true |
| IsScholarly | true |
| Issue | 1 |
| Language | English |
| License | Open Access This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License, which permits any non-commercial use, sharing, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if you modified the licensed material. You do not have permission under this licence to share adapted material derived from this article or parts of it. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by-nc-nd/4.0/. |
| LinkModel | DirectLink |
| MergedId | FETCHMERGED-LOGICAL-c3148-874e96dbe62819b565a253a36f30ba6434aadd8bb35f90110bd352ab400e72203 |
| Notes | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
| ORCID | 0000-0003-2461-1589 0000-0001-5051-9984 |
| OpenAccessLink | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11577015/ |
| PQID | 3130567921 |
| PQPubID | 546298 |
| PageCount | 18 |
| ParticipantIDs | doaj_primary_oai_doaj_org_article_e57ca63a79324d18b5975ab02734cb1b pubmedcentral_primary_oai_pubmedcentral_nih_gov_11577015 proquest_miscellaneous_3130830930 proquest_journals_3130567921 crossref_primary_10_1038_s41467_024_54260_8 springer_journals_10_1038_s41467_024_54260_8 |
| PublicationCentury | 2000 |
| PublicationDate | 11-19-2024 |
| PublicationDateYYYYMMDD | 2024-11-19 |
| PublicationDate_xml | – month: 11 year: 2024 text: 11-19-2024 day: 19 |
| PublicationDecade | 2020 |
| PublicationPlace | London |
| PublicationPlace_xml | – name: London |
| PublicationTitle | Nature communications |
| PublicationTitleAbbrev | Nat Commun |
| PublicationYear | 2024 |
| Publisher | Nature Publishing Group UK Nature Publishing Group Nature Portfolio |
| Publisher_xml | – name: Nature Publishing Group UK – name: Nature Publishing Group – name: Nature Portfolio |
| References | Stainbrook, Tyo (CR29) 2019; 103 Valley (CR48) 2012; 287 Cuthbertson, Nodwell (CR1) 2013; 77 Xu, Li, Zhang, Stephanopoulos, Koffas (CR10) 2014; 111 Mitchler, Garcia, Montero, Williams (CR4) 2021; 69 Silverman, Kelley-Loughnane, Lucks, Jewett (CR54) 2019; 8 Gaspar (CR66) 2018; 19 Galvão, Mencía, de Lorenzo (CR19) 2007; 65 Li, Zhang, Wu, Bai (CR28) 2020; 8 Acheson, Ho, Goularte, Cegelski, Zimmer (CR73) 2021; 28 Pedregosa (CR70) 2011; 12 Tang, Cirino (CR20) 2011; 50 Davis, van Dongen, Abreu-Goodger, Bartonicek, Enright (CR69) 2013; 63 Chen, Vishweshwaraiah, Dokholyan (CR24) 2022; 73 Vonrhein (CR75) 2011; 67 Raman, Rogers, Taylor, Church (CR12) 2014; 111 Leander, Yuan, Meger, Cui, Raman (CR22) 2020; 117 Weber, Warren (CR49) 2019; 672 Alford (CR35) 2017; 13 Buda, Miton, Fan, Tokuriki (CR31) 2023; 48 Punihaole (CR56) 2018; 122 CR5 Lien, Solheim, Ueland (CR39) 1991; 51 Dietrich, McKee, Keasling (CR8) 2010; 79 Fernandez-Escamilla, Fernandez-Ballester, Morel, Casares-Atienza, Ramos (CR60) 2015; 10 Mustafi, Grünberger, Kohlheyer, Bott, Frunzke (CR9) 2012; 14 d’Oelsnitz, Love, Diaz, Ellington (CR13) 2022; 11 Leaver-Fay (CR37) 2011; 487 CR47 Emsley, Cowtan (CR77) 2004; 60 Smith, Heger, Sudbery (CR68) 2017; 27 Huang (CR58) 2022; 11 Gelman, Fahlberg, Heinzelman, Romero, Gitter (CR45) 2021; 118 Peterman, Levine (CR26) 2016; 17 Kabsch (CR74) 2010; 66 Sharifi-Rad (CR42) 2022; 2022 Virtanen (CR71) 2020; 17 Wise, Kuske (CR18) 2000; 66 Jung (CR51) 2020; 38 Dauparas (CR57) 2022; 378 Hopf (CR62) 2017; 35 Novichkov (CR16) 2013; 14 Gong, Li, Cai, Stojkoska, Xie (CR2) 2019; 234 Li, Wang (CR52) 2020; 144 Tappin, Loughnane, McCarthy, Fitzsimons (CR55) 2016; 14 Nishikawa, Hoppe, Smith, Bingman, Raman (CR33) 2021; 12 Chen, Zhou, Chen, Gu (CR67) 2018; 34 Liu (CR50) 2020; 9 McInnes, Healy, Saul, Großberger (CR43) 2018; 3 Khersonsky (CR34) 2018; 72 Anderson, McKeown, Thornton (CR59) 2015; 4 Rohlhill, Sandoval, Papoutsakis (CR25) 2017; 6 Achan (CR40) 2011; 10 Niciu, Arias (CR41) 2013; 27 Liebschner (CR76) 2019; 75 Münch (CR15) 2003; 31 Riesselman, Ingraham, Marks (CR64) 2018; 15 Rogers, Taylor, Church (CR6) 2016; 42 Aharoni (CR30) 2005; 37 Dietrich, Shis, Alikhani, Keasling (CR11) 2013; 2 Süel, Lockless, Wall, Ranganathan (CR21) 2003; 10 Mahr, Frunzke (CR14) 2016; 100 Jha, Chakraborti, Kern, Fox, Strauss (CR36) 2015; 83 Kawashima, Ogata, Kanehisa (CR44) 1999; 27 Johnson (CR38) 2004; 85 Weerts (CR53) 2008; 33 Taylor (CR17) 2016; 13 Leander, Liu, Cui, Raman (CR23) 2022; 11 Alguel (CR32) 2007; 369 Pandurangan, Blundell (CR61) 2020; 29 CR63 Zhang, Carothers, Keasling (CR7) 2012; 30 Ding (CR27) 2020; 48 Kosuri (CR65) 2013; 110 Zhang, Kobert, Flouri, Stamatakis (CR72) 2014; 30 Meinhardt (CR3) 2012; 40 Jones (CR46) 2020; 9 AP Pandurangan (54260_CR61) 2020; 29 P Virtanen (54260_CR71) 2020; 17 54260_CR5 CC Valley (54260_CR48) 2012; 287 T Smith (54260_CR68) 2017; 27 JK Rogers (54260_CR6) 2016; 42 TC Galvão (54260_CR19) 2007; 65 F Zhang (54260_CR7) 2012; 30 J Achan (54260_CR40) 2011; 10 X Liu (54260_CR50) 2020; 9 M Leander (54260_CR23) 2022; 11 RF Alford (54260_CR35) 2017; 13 EM Weerts (54260_CR53) 2008; 33 S Kawashima (54260_CR44) 1999; 27 S Kosuri (54260_CR65) 2013; 110 EA Lien (54260_CR39) 1991; 51 54260_CR63 Z Li (54260_CR52) 2020; 144 S-Y Tang (54260_CR20) 2011; 50 AM Fernandez-Escamilla (54260_CR60) 2015; 10 GM Süel (54260_CR21) 2003; 10 S Raman (54260_CR12) 2014; 111 AA Wise (54260_CR18) 2000; 66 JA Dietrich (54260_CR8) 2010; 79 AD Silverman (54260_CR54) 2019; 8 A Aharoni (54260_CR30) 2005; 37 DS Weber (54260_CR49) 2019; 672 Y Alguel (54260_CR32) 2007; 369 J-W Li (54260_CR28) 2020; 8 S Gelman (54260_CR45) 2021; 118 54260_CR47 JK Jung (54260_CR51) 2020; 38 L Huang (54260_CR58) 2022; 11 EM Jones (54260_CR46) 2020; 9 F Pedregosa (54260_CR70) 2011; 12 MJ Niciu (54260_CR41) 2013; 27 N Mustafi (54260_CR9) 2012; 14 L McInnes (54260_CR43) 2018; 3 R Münch (54260_CR15) 2003; 31 AJ Riesselman (54260_CR64) 2018; 15 DW Anderson (54260_CR59) 2015; 4 K Buda (54260_CR31) 2023; 48 SC Stainbrook (54260_CR29) 2019; 103 JF Acheson (54260_CR73) 2021; 28 MD Johnson (54260_CR38) 2004; 85 S Chen (54260_CR67) 2018; 34 C Vonrhein (54260_CR75) 2011; 67 M Leander (54260_CR22) 2020; 117 J Sharifi-Rad (54260_CR42) 2022; 2022 RK Jha (54260_CR36) 2015; 83 AD Tappin (54260_CR55) 2016; 14 J Dauparas (54260_CR57) 2022; 378 PS Novichkov (54260_CR16) 2013; 14 A Leaver-Fay (54260_CR37) 2011; 487 D Liebschner (54260_CR76) 2019; 75 S d’Oelsnitz (54260_CR13) 2022; 11 J Chen (54260_CR24) 2022; 73 N Peterman (54260_CR26) 2016; 17 J Zhang (54260_CR72) 2014; 30 KK Nishikawa (54260_CR33) 2021; 12 JM Gaspar (54260_CR66) 2018; 19 JA Dietrich (54260_CR11) 2013; 2 R Mahr (54260_CR14) 2016; 100 Z Gong (54260_CR2) 2019; 234 S Meinhardt (54260_CR3) 2012; 40 MPA Davis (54260_CR69) 2013; 63 P Xu (54260_CR10) 2014; 111 ND Taylor (54260_CR17) 2016; 13 O Khersonsky (54260_CR34) 2018; 72 N Ding (54260_CR27) 2020; 48 J Rohlhill (54260_CR25) 2017; 6 L Cuthbertson (54260_CR1) 2013; 77 D Punihaole (54260_CR56) 2018; 122 MM Mitchler (54260_CR4) 2021; 69 TA Hopf (54260_CR62) 2017; 35 W Kabsch (54260_CR74) 2010; 66 P Emsley (54260_CR77) 2004; 60 |
| References_xml | – volume: 122 start-page: 9840 year: 2018 end-page: 9851 ident: CR56 article-title: New insights into quinine–DNA binding using Raman spectroscopy and molecular dynamics simulations publication-title: J. Phys. Chem. B doi: 10.1021/acs.jpcb.8b05795 contributor: fullname: Punihaole – volume: 65 start-page: 907 year: 2007 end-page: 919 ident: CR19 article-title: Emergence of novel functions in transcriptional regulators by regression to stem protein types publication-title: Mol. Microbiol. doi: 10.1111/j.1365-2958.2007.05832.x contributor: fullname: de Lorenzo – volume: 10 start-page: 59 year: 2003 end-page: 69 ident: CR21 article-title: Evolutionarily conserved networks of residues mediate allosteric communication in proteins publication-title: Nat. Struct. Biol. doi: 10.1038/nsb881 contributor: fullname: Ranganathan – volume: 40 start-page: 11139 year: 2012 end-page: 11154 ident: CR3 article-title: Novel insights from hybrid LacI/GalR proteins: family-wide functional attributes and biologically significant variation in transcription repression publication-title: Nucleic Acids Res. doi: 10.1093/nar/gks806 contributor: fullname: Meinhardt – volume: 37 start-page: 73 year: 2005 end-page: 76 ident: CR30 article-title: The “evolvability” of promiscuous protein functions publication-title: Nat. Genet. doi: 10.1038/ng1482 contributor: fullname: Aharoni – volume: 111 start-page: 11299 year: 2014 end-page: 11304 ident: CR10 article-title: Improving fatty acids production by engineering dynamic pathway regulation and metabolic control publication-title: Proc. Natl. Acad. Sci. USA doi: 10.1073/pnas.1406401111 contributor: fullname: Koffas – volume: 60 start-page: 2126 year: 2004 end-page: 2132 ident: CR77 article-title: Coot: model-building tools for molecular graphics publication-title: Acta Crystallogr. D Biol. Crystallogr. doi: 10.1107/S0907444904019158 contributor: fullname: Cowtan – volume: 17 start-page: 261 year: 2020 end-page: 272 ident: CR71 article-title: SciPy 1.0: fundamental algorithms for scientific computing in Python publication-title: Nat. Methods doi: 10.1038/s41592-019-0686-2 contributor: fullname: Virtanen – volume: 9 start-page: e54895 year: 2020 ident: CR46 article-title: Structural and functional characterization of G protein-coupled receptors with deep mutational scanning publication-title: eLife doi: 10.7554/eLife.54895 contributor: fullname: Jones – volume: 28 start-page: 310 year: 2021 end-page: 318 ident: CR73 article-title: Molecular organization of the E. Coli cellulose synthase macrocomplex publication-title: Nat. Struct. Mol. Biol. doi: 10.1038/s41594-021-00569-7 contributor: fullname: Zimmer – volume: 69 start-page: 172 year: 2021 end-page: 181 ident: CR4 article-title: Transcription factor-based biosensors: a molecular-guided approach for natural product engineering publication-title: Curr. Opin. Biotechnol. doi: 10.1016/j.copbio.2021.01.008 contributor: fullname: Williams – volume: 72 start-page: 178 year: 2018 end-page: 186.e5 ident: CR34 article-title: Automated design of efficient and functionally diverse enzyme repertoires publication-title: Mol. Cell doi: 10.1016/j.molcel.2018.08.033 contributor: fullname: Khersonsky – volume: 79 start-page: 563 year: 2010 end-page: 590 ident: CR8 article-title: High-throughput metabolic engineering: advances in small-molecule screening and selection publication-title: Annu. Rev. Biochem. doi: 10.1146/annurev-biochem-062608-095938 contributor: fullname: Keasling – volume: 85 start-page: 151 year: 2004 end-page: 159 ident: CR38 article-title: Pharmacological characterization of 4-hydroxy- desmethyl tamoxifen, a novel active metabolite of tamoxifen publication-title: Breast Cancer Res. Treat. doi: 10.1023/B:BREA.0000025406.31193.e8 contributor: fullname: Johnson – volume: 12 start-page: 2825 year: 2011 end-page: 2830 ident: CR70 article-title: Scikit-learn: machine learning in Python publication-title: J. Mach. Learn. Res. contributor: fullname: Pedregosa – volume: 2 start-page: 47 year: 2013 end-page: 58 ident: CR11 article-title: Transcription factor-based screens and synthetic selections for microbial small-molecule biosynthesis publication-title: ACS Synth. Biol. doi: 10.1021/sb300091d contributor: fullname: Keasling – volume: 67 start-page: 293 year: 2011 end-page: 302 ident: CR75 article-title: Data processing and analysis with the autoPROC toolbox publication-title: Acta Crystallogr. D Biol. Crystallogr. doi: 10.1107/S0907444911007773 contributor: fullname: Vonrhein – volume: 13 start-page: 177 year: 2016 end-page: 183 ident: CR17 article-title: Engineering an allosteric transcription factor to respond to new ligands publication-title: Nat. Methods doi: 10.1038/nmeth.3696 contributor: fullname: Taylor – volume: 11 start-page: 520 year: 2022 ident: CR58 article-title: Bacterial multidrug efflux pumps at the frontline of antimicrobial resistance: an overview publication-title: Antibiotics doi: 10.3390/antibiotics11040520 contributor: fullname: Huang – volume: 14 start-page: 449 year: 2012 end-page: 457 ident: CR9 article-title: The development and application of a single-cell biosensor for the detection of l-methionine and branched-chain amino acids publication-title: Metab. Eng. doi: 10.1016/j.ymben.2012.02.002 contributor: fullname: Frunzke – volume: 15 start-page: 816 year: 2018 end-page: 822 ident: CR64 article-title: Deep generative models of genetic variation capture the effects of mutations publication-title: Nat. Methods doi: 10.1038/s41592-018-0138-4 contributor: fullname: Marks – volume: 118 start-page: e2104878118 year: 2021 ident: CR45 article-title: Neural networks to learn protein sequence–function relationships from deep mutational scanning data publication-title: Proc. Natl. Acad. Sci. USA doi: 10.1073/pnas.2104878118 contributor: fullname: Gitter – volume: 672 start-page: 108053 year: 2019 ident: CR49 article-title: The interaction between methionine and two aromatic amino acids is an abundant and multifunctional motif in proteins publication-title: Arch. Biochem. Biophys. doi: 10.1016/j.abb.2019.07.018 contributor: fullname: Warren – volume: 48 start-page: 751 year: 2023 end-page: 760 ident: CR31 article-title: Molecular determinants of protein evolvability publication-title: Trends Biochem. Sci. doi: 10.1016/j.tibs.2023.05.009 contributor: fullname: Tokuriki – volume: 287 start-page: 34979 year: 2012 end-page: 34991 ident: CR48 article-title: The methionine-aromatic motif plays a unique role in stabilizing protein structure publication-title: J. Biol. Chem. doi: 10.1074/jbc.M112.374504 contributor: fullname: Valley – volume: 12 year: 2021 ident: CR33 article-title: Epistasis shapes the fitness landscape of an allosteric specificity switch publication-title: Nat. Commun. doi: 10.1038/s41467-021-25826-7 contributor: fullname: Raman – volume: 63 start-page: 41 year: 2013 end-page: 49 ident: CR69 article-title: Kraken: a set of tools for quality control and analysis of high-throughput sequence data publication-title: Methods doi: 10.1016/j.ymeth.2013.06.027 contributor: fullname: Enright – volume: 6 start-page: 1584 year: 2017 end-page: 1595 ident: CR25 article-title: Sort-seq approach to engineering a formaldehyde-inducible promoter for dynamically regulated escherichia coli growth on methanol publication-title: ACS Synth. Biol. doi: 10.1021/acssynbio.7b00114 contributor: fullname: Papoutsakis – ident: CR5 – volume: 42 start-page: 84 year: 2016 end-page: 91 ident: CR6 article-title: Biosensor-based engineering of biosynthetic pathways publication-title: Curr. Opin. Biotechnol. doi: 10.1016/j.copbio.2016.03.005 contributor: fullname: Church – volume: 117 start-page: 25445 year: 2020 end-page: 25454 ident: CR22 article-title: Functional plasticity and evolutionary adaptation of allosteric regulation publication-title: Proc. Natl. Acad. Sci. USA doi: 10.1073/pnas.2002613117 contributor: fullname: Raman – volume: 29 start-page: 247 year: 2020 end-page: 257 ident: CR61 article-title: Prediction of Impacts of mutations on protein structure and interactions: SDM, a Statistical approach, and mCSM, using machine learning publication-title: Protein Sci. doi: 10.1002/pro.3774 contributor: fullname: Blundell – volume: 75 start-page: 861 year: 2019 end-page: 877 ident: CR76 article-title: Macromolecular structure determination using X-rays, neutrons and electrons: recent developments in Phenix publication-title: Acta Crystallogr. D Struct. Biol. doi: 10.1107/S2059798319011471 contributor: fullname: Liebschner – volume: 66 start-page: 163 year: 2000 end-page: 169 ident: CR18 article-title: Generation of novel bacterial regulatory proteins that detect priority pollutant phenols publication-title: Appl. Environ. Microbiol. doi: 10.1128/AEM.66.1.163-169.2000 contributor: fullname: Kuske – volume: 234 start-page: 19237 year: 2019 end-page: 19248 ident: CR2 article-title: Biology of MarR family transcription factors and implications for targets of antibiotics against tuberculosis publication-title: J. Cell. Physiol. doi: 10.1002/jcp.28720 contributor: fullname: Xie – volume: 48 start-page: 10602 year: 2020 end-page: 10613 ident: CR27 article-title: Programmable cross-ribosome-binding sites to fine-tune the dynamic range of transcription factor-based biosensor publication-title: Nucleic Acids Res. doi: 10.1093/nar/gkaa786 contributor: fullname: Ding – volume: 19 year: 2018 ident: CR66 article-title: NGmerge: merging paired-end reads via novel empirically-derived models of sequencing errors publication-title: BMC Bioinform. doi: 10.1186/s12859-018-2579-2 contributor: fullname: Gaspar – volume: 17 year: 2016 ident: CR26 article-title: Sort-seq under the hood: implications of design choices on large-scale characterization of sequence-function relations publication-title: BMC Genom. doi: 10.1186/s12864-016-2533-5 contributor: fullname: Levine – ident: CR47 – volume: 27 start-page: 368 year: 1999 end-page: 369 ident: CR44 article-title: AAindex: amino acid index database publication-title: Nucleic Acids Res. doi: 10.1093/nar/27.1.368 contributor: fullname: Kanehisa – volume: 111 start-page: 17803 year: 2014 end-page: 17808 ident: CR12 article-title: Evolution-guided optimization of biosynthetic pathways publication-title: Proc. Natl. Acad. Sci. USA doi: 10.1073/pnas.1409523111 contributor: fullname: Church – volume: 487 start-page: 545 year: 2011 end-page: 574 ident: CR37 article-title: ROSETTA3: an object-oriented software suite for the simulation and design of macromolecules publication-title: Methods Enzymol. doi: 10.1016/B978-0-12-381270-4.00019-6 contributor: fullname: Leaver-Fay – volume: 2022 start-page: e3848084 year: 2022 ident: CR42 article-title: Ellagic acid: a review on its natural sources, chemical stability, and therapeutic potential publication-title: Oxid. Med. Cell. Longev. doi: 10.1155/2022/3848084 contributor: fullname: Sharifi-Rad – volume: 3 start-page: 861 year: 2018 ident: CR43 article-title: UMAP: uniform manifold approximation and projection publication-title: J. Open Source Softw. doi: 10.21105/joss.00861 contributor: fullname: Großberger – volume: 9 start-page: 84 year: 2020 end-page: 94 ident: CR50 article-title: Design of a transcriptional biosensor for the portable, on-demand detection of cyanuric acid publication-title: ACS Synth. Biol. doi: 10.1021/acssynbio.9b00348 contributor: fullname: Liu – volume: 144 start-page: 1325 year: 2020 end-page: 1334 ident: CR52 article-title: Point-of-care drug of abuse testing in the opioid epidemic publication-title: Arch. Pathol. Lab. Med. doi: 10.5858/arpa.2020-0055-RA contributor: fullname: Wang – volume: 50 start-page: 1084 year: 2011 end-page: 1086 ident: CR20 article-title: Design and application of a mevalonate-responsive regulatory protein publication-title: Angew. Chem. Int. Ed. Engl. doi: 10.1002/anie.201006083 contributor: fullname: Cirino – volume: 10 year: 2011 ident: CR40 article-title: Quinine, an old anti-malarial drug in a modern world: role in the treatment of malaria publication-title: Malar. J. doi: 10.1186/1475-2875-10-144 contributor: fullname: Achan – volume: 378 start-page: 49 year: 2022 end-page: 56 ident: CR57 article-title: Robust deep learning-based protein sequence design using ProteinMPNN publication-title: Science doi: 10.1126/science.add2187 contributor: fullname: Dauparas – volume: 110 start-page: 14024 year: 2013 end-page: 14029 ident: CR65 article-title: Composability of regulatory sequences controlling transcription and translation in Escherichia Coli publication-title: Proc. Natl. Acad. Sci. USA doi: 10.1073/pnas.1301301110 contributor: fullname: Kosuri – ident: CR63 – volume: 369 start-page: 829 year: 2007 end-page: 840 ident: CR32 article-title: Crystal structures of multidrug binding protein TtgR in complex with antibiotics and plant antimicrobials publication-title: J. Mol. Biol. doi: 10.1016/j.jmb.2007.03.062 contributor: fullname: Alguel – volume: 83 start-page: 1327 year: 2015 end-page: 1340 ident: CR36 article-title: Rosetta comparative modeling for library design: engineering alternative inducer specificity in a transcription factor publication-title: Proteins doi: 10.1002/prot.24828 contributor: fullname: Strauss – volume: 35 start-page: 128 year: 2017 end-page: 135 ident: CR62 article-title: Mutation effects predicted from sequence co-variation publication-title: Nat. Biotechnol. doi: 10.1038/nbt.3769 contributor: fullname: Hopf – volume: 31 start-page: 266 year: 2003 end-page: 269 ident: CR15 article-title: PRODORIC: prokaryotic database of gene regulation publication-title: Nucleic Acids Res. doi: 10.1093/nar/gkg037 contributor: fullname: Münch – volume: 100 start-page: 79 year: 2016 end-page: 90 ident: CR14 article-title: Transcription factor-based biosensors in biotechnology: current state and future prospects publication-title: Appl. Microbiol. Biotechnol. doi: 10.1007/s00253-015-7090-3 contributor: fullname: Frunzke – volume: 73 start-page: 102334 year: 2022 ident: CR24 article-title: Design and engineering of allosteric communications in proteins publication-title: Curr. Opin. Struct. Biol. doi: 10.1016/j.sbi.2022.102334 contributor: fullname: Dokholyan – volume: 8 start-page: 403 year: 2019 end-page: 414 ident: CR54 article-title: Deconstructing cell-free extract preparation for in vitro activation of transcriptional genetic circuitry publication-title: ACS Synth. Biol. doi: 10.1021/acssynbio.8b00430 contributor: fullname: Jewett – volume: 11 start-page: e79932 year: 2022 ident: CR23 article-title: Deep mutational scanning and machine learning reveal structural and molecular rules governing allosteric hotspots in homologous proteins publication-title: eLife doi: 10.7554/eLife.79932 contributor: fullname: Raman – volume: 30 start-page: 354 year: 2012 end-page: 359 ident: CR7 article-title: Design of a dynamic sensor-regulator system for production of chemicals and fuels derived from fatty acids publication-title: Nat. Biotechnol. doi: 10.1038/nbt.2149 contributor: fullname: Keasling – volume: 77 start-page: 440 year: 2013 end-page: 475 ident: CR1 article-title: The TetR family of regulators publication-title: Microbiol. Mol. Biol. Rev. doi: 10.1128/MMBR.00018-13 contributor: fullname: Nodwell – volume: 11 start-page: 3534 year: 2022 end-page: 3537 ident: CR13 article-title: GroovDB: a database of ligand-inducible transcription factors publication-title: ACS Synth. Biol. doi: 10.1021/acssynbio.2c00382 contributor: fullname: Ellington – volume: 13 start-page: 3031 year: 2017 end-page: 3048 ident: CR35 article-title: The Rosetta all-atom energy function for macromolecular modeling and design publication-title: J. Chem. Theory Comput. doi: 10.1021/acs.jctc.7b00125 contributor: fullname: Alford – volume: 51 start-page: 4837 year: 1991 end-page: 4844 ident: CR39 article-title: Distribution of tamoxifen and its metabolites in rat and human tissues during steady-state treatment publication-title: Cancer Res. contributor: fullname: Ueland – volume: 103 start-page: 9697 year: 2019 end-page: 9709 ident: CR29 article-title: Model-guided mechanism discovery and parameter selection for directed evolution publication-title: Appl. Microbiol. Biotechnol. doi: 10.1007/s00253-019-10179-5 contributor: fullname: Tyo – volume: 10 start-page: e0138469 year: 2015 ident: CR60 article-title: Molecular binding mechanism of TtgR repressor to antibiotics and antimicrobials publication-title: PLoS ONE doi: 10.1371/journal.pone.0138469 contributor: fullname: Ramos – volume: 8 start-page: 98 year: 2020 ident: CR28 article-title: Transcription factor engineering for high-throughput strain evolution and organic acid bioproduction: a review publication-title: Front. Bioeng. Biotechnol. doi: 10.3389/fbioe.2020.00098 contributor: fullname: Bai – volume: 27 start-page: 777 year: 2013 end-page: 787 ident: CR41 article-title: Targeted opioid receptor antagonists in the treatment of alcohol use disorders publication-title: CNS Drugs doi: 10.1007/s40263-013-0096-4 contributor: fullname: Arias – volume: 14 start-page: 455 year: 2016 end-page: 465 ident: CR55 article-title: Unexpected removal of the most neutral cationic pharmaceutical in river waters publication-title: Environ. Chem. Lett. doi: 10.1007/s10311-016-0582-2 contributor: fullname: Fitzsimons – volume: 30 start-page: 614 year: 2014 end-page: 620 ident: CR72 article-title: PEAR: a fast and accurate Illumina Paired-End reAd mergeR publication-title: Bioinformatics doi: 10.1093/bioinformatics/btt593 contributor: fullname: Stamatakis – volume: 38 start-page: 1451 year: 2020 end-page: 1459 ident: CR51 article-title: Cell-free biosensors for rapid detection of water contaminants publication-title: Nat. Biotechnol. doi: 10.1038/s41587-020-0571-7 contributor: fullname: Jung – volume: 4 start-page: e07864 year: 2015 ident: CR59 article-title: Intermolecular epistasis shaped the function and evolution of an ancient transcription factor and its DNA binding sites publication-title: eLife doi: 10.7554/eLife.07864 contributor: fullname: Thornton – volume: 34 start-page: i884 year: 2018 end-page: i890 ident: CR67 article-title: Fastp: an ultra-fast all-in-one FASTQ preprocessor publication-title: Bioinformatics doi: 10.1093/bioinformatics/bty560 contributor: fullname: Gu – volume: 14 year: 2013 ident: CR16 article-title: RegPrecise 3.0—a resource for genome-scale exploration of transcriptional regulation in bacteria publication-title: BMC Genom. doi: 10.1186/1471-2164-14-745 contributor: fullname: Novichkov – volume: 66 start-page: 125 year: 2010 end-page: 132 ident: CR74 article-title: XDS publication-title: Acta Crystallogr. D Biol. Crystallogr. doi: 10.1107/S0907444909047337 contributor: fullname: Kabsch – volume: 33 start-page: 653 year: 2008 end-page: 665 ident: CR53 article-title: Differences in delta- and mu-opioid receptor blockade measured by positron emission tomography in naltrexone-treated recently abstinent alcohol-dependent subjects publication-title: Neuropsychopharmacology doi: 10.1038/sj.npp.1301440 contributor: fullname: Weerts – volume: 27 start-page: 491 year: 2017 end-page: 499 ident: CR68 article-title: UMI-tools: modeling sequencing errors in unique molecular identifiers to improve quantification accuracy publication-title: Genome Res. doi: 10.1101/gr.209601.116 contributor: fullname: Sudbery – volume: 11 start-page: 3534 year: 2022 ident: 54260_CR13 publication-title: ACS Synth. Biol. doi: 10.1021/acssynbio.2c00382 contributor: fullname: S d’Oelsnitz – volume: 66 start-page: 163 year: 2000 ident: 54260_CR18 publication-title: Appl. Environ. Microbiol. doi: 10.1128/AEM.66.1.163-169.2000 contributor: fullname: AA Wise – volume: 4 start-page: e07864 year: 2015 ident: 54260_CR59 publication-title: eLife doi: 10.7554/eLife.07864 contributor: fullname: DW Anderson – volume: 111 start-page: 11299 year: 2014 ident: 54260_CR10 publication-title: Proc. Natl. Acad. Sci. USA doi: 10.1073/pnas.1406401111 contributor: fullname: P Xu – volume: 29 start-page: 247 year: 2020 ident: 54260_CR61 publication-title: Protein Sci. doi: 10.1002/pro.3774 contributor: fullname: AP Pandurangan – volume: 63 start-page: 41 year: 2013 ident: 54260_CR69 publication-title: Methods doi: 10.1016/j.ymeth.2013.06.027 contributor: fullname: MPA Davis – volume: 60 start-page: 2126 year: 2004 ident: 54260_CR77 publication-title: Acta Crystallogr. D Biol. Crystallogr. doi: 10.1107/S0907444904019158 contributor: fullname: P Emsley – volume: 27 start-page: 777 year: 2013 ident: 54260_CR41 publication-title: CNS Drugs doi: 10.1007/s40263-013-0096-4 contributor: fullname: MJ Niciu – volume: 3 start-page: 861 year: 2018 ident: 54260_CR43 publication-title: J. Open Source Softw. doi: 10.21105/joss.00861 contributor: fullname: L McInnes – volume: 33 start-page: 653 year: 2008 ident: 54260_CR53 publication-title: Neuropsychopharmacology doi: 10.1038/sj.npp.1301440 contributor: fullname: EM Weerts – volume: 69 start-page: 172 year: 2021 ident: 54260_CR4 publication-title: Curr. Opin. Biotechnol. doi: 10.1016/j.copbio.2021.01.008 contributor: fullname: MM Mitchler – volume: 10 start-page: e0138469 year: 2015 ident: 54260_CR60 publication-title: PLoS ONE doi: 10.1371/journal.pone.0138469 contributor: fullname: AM Fernandez-Escamilla – volume: 19 year: 2018 ident: 54260_CR66 publication-title: BMC Bioinform. doi: 10.1186/s12859-018-2579-2 contributor: fullname: JM Gaspar – volume: 83 start-page: 1327 year: 2015 ident: 54260_CR36 publication-title: Proteins doi: 10.1002/prot.24828 contributor: fullname: RK Jha – volume: 66 start-page: 125 year: 2010 ident: 54260_CR74 publication-title: Acta Crystallogr. D Biol. Crystallogr. doi: 10.1107/S0907444909047337 contributor: fullname: W Kabsch – volume: 2 start-page: 47 year: 2013 ident: 54260_CR11 publication-title: ACS Synth. Biol. doi: 10.1021/sb300091d contributor: fullname: JA Dietrich – volume: 14 start-page: 455 year: 2016 ident: 54260_CR55 publication-title: Environ. Chem. Lett. doi: 10.1007/s10311-016-0582-2 contributor: fullname: AD Tappin – volume: 8 start-page: 403 year: 2019 ident: 54260_CR54 publication-title: ACS Synth. Biol. doi: 10.1021/acssynbio.8b00430 contributor: fullname: AD Silverman – volume: 9 start-page: 84 year: 2020 ident: 54260_CR50 publication-title: ACS Synth. Biol. doi: 10.1021/acssynbio.9b00348 contributor: fullname: X Liu – volume: 30 start-page: 614 year: 2014 ident: 54260_CR72 publication-title: Bioinformatics doi: 10.1093/bioinformatics/btt593 contributor: fullname: J Zhang – ident: 54260_CR5 doi: 10.3389/fbioe.2023.1118702 – volume: 67 start-page: 293 year: 2011 ident: 54260_CR75 publication-title: Acta Crystallogr. D Biol. Crystallogr. doi: 10.1107/S0907444911007773 contributor: fullname: C Vonrhein – volume: 122 start-page: 9840 year: 2018 ident: 54260_CR56 publication-title: J. Phys. Chem. B doi: 10.1021/acs.jpcb.8b05795 contributor: fullname: D Punihaole – volume: 11 start-page: 520 year: 2022 ident: 54260_CR58 publication-title: Antibiotics doi: 10.3390/antibiotics11040520 contributor: fullname: L Huang – volume: 15 start-page: 816 year: 2018 ident: 54260_CR64 publication-title: Nat. Methods doi: 10.1038/s41592-018-0138-4 contributor: fullname: AJ Riesselman – volume: 17 start-page: 261 year: 2020 ident: 54260_CR71 publication-title: Nat. Methods doi: 10.1038/s41592-019-0686-2 contributor: fullname: P Virtanen – volume: 10 start-page: 59 year: 2003 ident: 54260_CR21 publication-title: Nat. Struct. Biol. doi: 10.1038/nsb881 contributor: fullname: GM Süel – volume: 79 start-page: 563 year: 2010 ident: 54260_CR8 publication-title: Annu. Rev. Biochem. doi: 10.1146/annurev-biochem-062608-095938 contributor: fullname: JA Dietrich – volume: 369 start-page: 829 year: 2007 ident: 54260_CR32 publication-title: J. Mol. Biol. doi: 10.1016/j.jmb.2007.03.062 contributor: fullname: Y Alguel – volume: 27 start-page: 368 year: 1999 ident: 54260_CR44 publication-title: Nucleic Acids Res. doi: 10.1093/nar/27.1.368 contributor: fullname: S Kawashima – ident: 54260_CR47 doi: 10.1007/978-3-642-37456-2_14 – volume: 17 year: 2016 ident: 54260_CR26 publication-title: BMC Genom. doi: 10.1186/s12864-016-2533-5 contributor: fullname: N Peterman – volume: 34 start-page: i884 year: 2018 ident: 54260_CR67 publication-title: Bioinformatics doi: 10.1093/bioinformatics/bty560 contributor: fullname: S Chen – volume: 8 start-page: 98 year: 2020 ident: 54260_CR28 publication-title: Front. Bioeng. Biotechnol. doi: 10.3389/fbioe.2020.00098 contributor: fullname: J-W Li – ident: 54260_CR63 doi: 10.1002/pro.4780 – volume: 10 year: 2011 ident: 54260_CR40 publication-title: Malar. J. doi: 10.1186/1475-2875-10-144 contributor: fullname: J Achan – volume: 13 start-page: 3031 year: 2017 ident: 54260_CR35 publication-title: J. Chem. Theory Comput. doi: 10.1021/acs.jctc.7b00125 contributor: fullname: RF Alford – volume: 6 start-page: 1584 year: 2017 ident: 54260_CR25 publication-title: ACS Synth. Biol. doi: 10.1021/acssynbio.7b00114 contributor: fullname: J Rohlhill – volume: 110 start-page: 14024 year: 2013 ident: 54260_CR65 publication-title: Proc. Natl. Acad. Sci. USA doi: 10.1073/pnas.1301301110 contributor: fullname: S Kosuri – volume: 48 start-page: 751 year: 2023 ident: 54260_CR31 publication-title: Trends Biochem. Sci. doi: 10.1016/j.tibs.2023.05.009 contributor: fullname: K Buda – volume: 48 start-page: 10602 year: 2020 ident: 54260_CR27 publication-title: Nucleic Acids Res. doi: 10.1093/nar/gkaa786 contributor: fullname: N Ding – volume: 27 start-page: 491 year: 2017 ident: 54260_CR68 publication-title: Genome Res. doi: 10.1101/gr.209601.116 contributor: fullname: T Smith – volume: 111 start-page: 17803 year: 2014 ident: 54260_CR12 publication-title: Proc. Natl. Acad. Sci. USA doi: 10.1073/pnas.1409523111 contributor: fullname: S Raman – volume: 12 year: 2021 ident: 54260_CR33 publication-title: Nat. Commun. doi: 10.1038/s41467-021-25826-7 contributor: fullname: KK Nishikawa – volume: 77 start-page: 440 year: 2013 ident: 54260_CR1 publication-title: Microbiol. Mol. Biol. Rev. doi: 10.1128/MMBR.00018-13 contributor: fullname: L Cuthbertson – volume: 12 start-page: 2825 year: 2011 ident: 54260_CR70 publication-title: J. Mach. Learn. Res. contributor: fullname: F Pedregosa – volume: 38 start-page: 1451 year: 2020 ident: 54260_CR51 publication-title: Nat. Biotechnol. doi: 10.1038/s41587-020-0571-7 contributor: fullname: JK Jung – volume: 35 start-page: 128 year: 2017 ident: 54260_CR62 publication-title: Nat. Biotechnol. doi: 10.1038/nbt.3769 contributor: fullname: TA Hopf – volume: 73 start-page: 102334 year: 2022 ident: 54260_CR24 publication-title: Curr. Opin. Struct. Biol. doi: 10.1016/j.sbi.2022.102334 contributor: fullname: J Chen – volume: 144 start-page: 1325 year: 2020 ident: 54260_CR52 publication-title: Arch. Pathol. Lab. Med. doi: 10.5858/arpa.2020-0055-RA contributor: fullname: Z Li – volume: 13 start-page: 177 year: 2016 ident: 54260_CR17 publication-title: Nat. Methods doi: 10.1038/nmeth.3696 contributor: fullname: ND Taylor – volume: 72 start-page: 178 year: 2018 ident: 54260_CR34 publication-title: Mol. Cell doi: 10.1016/j.molcel.2018.08.033 contributor: fullname: O Khersonsky – volume: 14 start-page: 449 year: 2012 ident: 54260_CR9 publication-title: Metab. Eng. doi: 10.1016/j.ymben.2012.02.002 contributor: fullname: N Mustafi – volume: 378 start-page: 49 year: 2022 ident: 54260_CR57 publication-title: Science doi: 10.1126/science.add2187 contributor: fullname: J Dauparas – volume: 100 start-page: 79 year: 2016 ident: 54260_CR14 publication-title: Appl. Microbiol. Biotechnol. doi: 10.1007/s00253-015-7090-3 contributor: fullname: R Mahr – volume: 65 start-page: 907 year: 2007 ident: 54260_CR19 publication-title: Mol. Microbiol. doi: 10.1111/j.1365-2958.2007.05832.x contributor: fullname: TC Galvão – volume: 117 start-page: 25445 year: 2020 ident: 54260_CR22 publication-title: Proc. Natl. Acad. Sci. USA doi: 10.1073/pnas.2002613117 contributor: fullname: M Leander – volume: 30 start-page: 354 year: 2012 ident: 54260_CR7 publication-title: Nat. Biotechnol. doi: 10.1038/nbt.2149 contributor: fullname: F Zhang – volume: 51 start-page: 4837 year: 1991 ident: 54260_CR39 publication-title: Cancer Res. contributor: fullname: EA Lien – volume: 37 start-page: 73 year: 2005 ident: 54260_CR30 publication-title: Nat. Genet. doi: 10.1038/ng1482 contributor: fullname: A Aharoni – volume: 487 start-page: 545 year: 2011 ident: 54260_CR37 publication-title: Methods Enzymol. doi: 10.1016/B978-0-12-381270-4.00019-6 contributor: fullname: A Leaver-Fay – volume: 14 year: 2013 ident: 54260_CR16 publication-title: BMC Genom. doi: 10.1186/1471-2164-14-745 contributor: fullname: PS Novichkov – volume: 118 start-page: e2104878118 year: 2021 ident: 54260_CR45 publication-title: Proc. Natl. Acad. Sci. USA doi: 10.1073/pnas.2104878118 contributor: fullname: S Gelman – volume: 672 start-page: 108053 year: 2019 ident: 54260_CR49 publication-title: Arch. Biochem. Biophys. doi: 10.1016/j.abb.2019.07.018 contributor: fullname: DS Weber – volume: 40 start-page: 11139 year: 2012 ident: 54260_CR3 publication-title: Nucleic Acids Res. doi: 10.1093/nar/gks806 contributor: fullname: S Meinhardt – volume: 103 start-page: 9697 year: 2019 ident: 54260_CR29 publication-title: Appl. Microbiol. Biotechnol. doi: 10.1007/s00253-019-10179-5 contributor: fullname: SC Stainbrook – volume: 85 start-page: 151 year: 2004 ident: 54260_CR38 publication-title: Breast Cancer Res. Treat. doi: 10.1023/B:BREA.0000025406.31193.e8 contributor: fullname: MD Johnson – volume: 28 start-page: 310 year: 2021 ident: 54260_CR73 publication-title: Nat. Struct. Mol. Biol. doi: 10.1038/s41594-021-00569-7 contributor: fullname: JF Acheson – volume: 50 start-page: 1084 year: 2011 ident: 54260_CR20 publication-title: Angew. Chem. Int. Ed. Engl. doi: 10.1002/anie.201006083 contributor: fullname: S-Y Tang – volume: 9 start-page: e54895 year: 2020 ident: 54260_CR46 publication-title: eLife doi: 10.7554/eLife.54895 contributor: fullname: EM Jones – volume: 75 start-page: 861 year: 2019 ident: 54260_CR76 publication-title: Acta Crystallogr. D Struct. Biol. doi: 10.1107/S2059798319011471 contributor: fullname: D Liebschner – volume: 2022 start-page: e3848084 year: 2022 ident: 54260_CR42 publication-title: Oxid. Med. Cell. Longev. doi: 10.1155/2022/3848084 contributor: fullname: J Sharifi-Rad – volume: 234 start-page: 19237 year: 2019 ident: 54260_CR2 publication-title: J. Cell. Physiol. doi: 10.1002/jcp.28720 contributor: fullname: Z Gong – volume: 11 start-page: e79932 year: 2022 ident: 54260_CR23 publication-title: eLife doi: 10.7554/eLife.79932 contributor: fullname: M Leander – volume: 287 start-page: 34979 year: 2012 ident: 54260_CR48 publication-title: J. Biol. Chem. doi: 10.1074/jbc.M112.374504 contributor: fullname: CC Valley – volume: 31 start-page: 266 year: 2003 ident: 54260_CR15 publication-title: Nucleic Acids Res. doi: 10.1093/nar/gkg037 contributor: fullname: R Münch – volume: 42 start-page: 84 year: 2016 ident: 54260_CR6 publication-title: Curr. Opin. Biotechnol. doi: 10.1016/j.copbio.2016.03.005 contributor: fullname: JK Rogers |
| SSID | ssj0000391844 |
| Score | 2.5042727 |
| Snippet | Allosteric transcription factors (aTF) regulate gene expression through conformational changes induced by small molecule binding. Although widely used as... Abstract Allosteric transcription factors (aTF) regulate gene expression through conformational changes induced by small molecule binding. Although widely used... |
| SourceID | doaj pubmedcentral proquest crossref springer |
| SourceType | Open Website Open Access Repository Aggregation Database Publisher |
| StartPage | 10001 |
| SubjectTerms | 49 49/23 631/1647/1888 631/326/2522 631/45/612/822 631/61/338/469 Allosteric properties Antimalarial agents Binding Biosensors Design Design factors Flavonoids Gene expression Humanities and Social Sciences Ligands Methionine multidisciplinary Naltrexone Naringenin Quinine Science Science (multidisciplinary) Sensors Transcription factors |
| SummonAdditionalLinks | – databaseName: Directory of Open Access Journals dbid: DOA link: http://sdu.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwrV3NS-wwEB9UELyIn7z6RQRvz2LTpGl69GvZkxcVvIV89b0F6Yq7C_rfm0nbfVZ4ePHaFJrMTOajM_MbgDNNTeWEL9KMe5FybmxaWcrSXOiaUucEFdgoPL4v757kzS3C5CxHfWFNWAsP3BLuwhel1YLpIEc5d1Sa4AEX2kRYFmuoido3E5-CqaiDWRVCF951yWRMXsx41AnBJKUForKncmCJImD_wMv8WiP5JVEa7c9oCzY7x5FcthvehhXf7MB6O0ryfRcesGDj-Z30BYJv3hEXqzPItCa6IZhgR1CEiSVztE-9tiDtxB0yn5JZCGk9CX42eZ78wRbgPXgc3T5cj9NuYkJqGf4ZlCX3lXDGC8yPmeCs6bxgmomaZUYH54ProM-kMayosec0My44YNqEi-zLPM_YPqw108b_AlIWtGa88lRbx7UrZRxNbYOJ14WvvE3gd0899dICY6iY0GZStbRWgdYq0lrJBK6QwMs3EdQ6PgisVh2r1XesTuCoZ4_qbtpMMYpBUFnlNIHT5XK4I5j40I2fLtp3JKZ8swTkgK2DDQ1XmsnfiLaNaERlcJoSOO8l4N_X_3_ig5848SFs5CixWHZYHcHa_HXhj2F15hYnUd4_AHh8Anw priority: 102 providerName: Directory of Open Access Journals |
| Title | Highly multiplexed design of an allosteric transcription factor to sense new ligands |
| URI | https://link.springer.com/article/10.1038/s41467-024-54260-8 https://www.proquest.com/docview/3130567921 https://www.proquest.com/docview/3130830930 https://pubmed.ncbi.nlm.nih.gov/PMC11577015 https://doaj.org/article/e57ca63a79324d18b5975ab02734cb1b |
| Volume | 15 |
| hasFullText | 1 |
| inHoldings | 1 |
| isFullTextHit | |
| isPrint | |
| link | http://sdu.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1Lb9QwEB6xlZC4IJ4iUCojcYN049hxnGMprXoBIVEkbpZfKSttk6q7K9F_3xknKaQSF66xJTvj8Tw8M98AvLfcNUHFKi9kVLmUzueN5yIvlW05D0FxRYXCZ9_rrz_15xOCyVFTLUxK2vdudditLw-71a-UW3l16ZdTntjy25djAoipUY8tF7BA4_AvHz3JX9Gg2yLHCplC6OVGJnmA6iivCJE91zMtlMD6Zxbm_fzIe0HSpHtOn8Dj0WhkR8PmnsKD2D2Dh0MbyZvncE7JGusbNiUH_o6BhZSZwfqW2Y5RcJ0AEVaebUk3TZKCDd122LZnG3RnI0Mbm61XF1T--wJ-nJ6cH5_lY7eE3At6FdS1jI0KLiqKjTk01GxZCStUKwpn0fCQFmWZdk5ULdWbFi6g8WUdXuJYl2UhXsJe13fxFbC64q2QTeTWB2lDrVNbao_q3VaxiT6DDxP1zNUAimFSMFtoM9DaIK1NorXRGXwiAt_NJEDr9KG_vjDjsZpY1d4qYVFclDJw7dDRqaxL6DvecZfB_nQ8ZrxlGyM4OUB1U_IM3t0N4_2goIftYr8b5mgK9xYZ6NmxzjY0H0HGS0jbE6Nl8HHigD-r__uPX___Sm_gUUl8SomGzT7sba938S0sNmF3kJ4LDhKv3wKSywLn |
| link.rule.ids | 230,315,729,782,786,866,887,2106,27933,27934,53800,53802 |
| linkProvider | National Library of Medicine |
| linkToHtml | http://sdu.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1Lb9QwEB7RIgQX3oj0AUbiBunGseM4x1JaLaKtkFgkbpZfKSttk6q7K7X_Ho-TlKYSl17jSHY8nlfmm88AHzU1lRO-SDPuRcq5sWllKUtzoWtKnRNUYKPw9Gd5-lt-PUSaHDH0wkTQvjXzvWZxvtfM_0Rs5cW5nQw4scmPkwMkiCmDH5tswMOgsFl2K0uPFphVIXHhfY9MxuRkyaNFCA4pLZCTPZUjPxTp-kcx5l2E5J0yafQ-R8_uu-7n8LSPN8l-N_4CHvjmJTzqbqC8fgUzxHksrsmAK7zyjrgI6iBtTXRDsC6PXApzS1bo1gYjQ7qLesiqJcuQCXsSwnOymJ9h5_Br-HV0ODuYpv1FC6ll-ENRltxXwhkvsKxmQoyn84JpJmqWGR1iFq6DGZTGsKLGVtXMuBC3aRP035d5nrE3sNm0jX8LpCxozXjlqbaOa1fKeKO1DZGBLnzlbQKfhm1XFx2fhop1cCZVJyQVhKSikJRM4AtK5uZN5MKOD9rLM9XvrPJFabVgOlianDsqTciRCm0icY811CSwM8hV9Qq6VIxi7lRWOU3gw81wUC2sl-jGt-vuHYmV4iwBOToPowWNR4LsI0n3IOsEPg9H59_s___irfvP9B4eT2cnx-r42-n3bXiS42FHvGK1A5ury7XfhY2lW7-LqvIXyhwXpg |
| linkToPdf | http://sdu.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwpV1Lb9QwEB7RIhAX3hWBAkbiBmni2HEcbtB2VQRUlSgSN8uvlJW2yaq7K9F_j8dJCqnEBa6xJTsZzyvzzWeA15qa2glfpjn3IuXc2LS2lKWF0A2lzgkqsFH46Gt1_F0eHCJNzruxFyaC9q2Z77WL8712_iNiK5fnNhtxYtnJl30kiKmCH8uWrsm24GZQ2rz4I1OPVpjVIXnhQ59MzmS24tEqBKeUlsjLnsqJL4qU_ZM48zpK8lqpNHqg2b3_2ft9uDvEneR9P-cB3PDtQ7jV30R5-QhOEe-xuCQjvvCnd8RFcAfpGqJbgvV55FSYW7JG9zYaG9Jf2EPWHVmFjNiTEKaTxfwMO4gfw7fZ4en-UTpcuJBahj8WZcV9LZzxAstrJsR6uiiZZqJhudEhduE6mENpDCsbbFnNjQvxmzbBDviqKHK2A9tt1_onQKqSNozXnmrruHaVjDdb2xAh6NLX3ibwZvz0atnzaqhYD2dS9YJSQVAqCkrJBD6gdK5mIid2fNBdnKnh6ypfVlYLpoPFKbij0oRcqdQmEvhYQ00Cu6Ns1aCoK8Uo5lBVXdAEXl0NBxXDuolufbfp50isGOcJyMmZmGxoOhLkH8m6R3kn8HY8Pr9X__sbP_33lV7C7ZODmfr88fjTM7hT4HlH2GK9C9vri41_Dlsrt3kRteUXoMkaJg |
| openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Highly+multiplexed+design+of+an+allosteric+transcription+factor+to+sense+new+ligands&rft.jtitle=Nature+communications&rft.au=Nishikawa%2C+Kyle+K.&rft.au=Chen%2C+Jackie&rft.au=Acheson%2C+Justin+F.&rft.au=Harbaugh%2C+Svetlana+V.&rft.date=2024-11-19&rft.pub=Nature+Publishing+Group+UK&rft.eissn=2041-1723&rft.volume=15&rft.issue=1&rft_id=info:doi/10.1038%2Fs41467-024-54260-8&rft.externalDocID=10_1038_s41467_024_54260_8 |
| thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=2041-1723&client=summon |
| thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=2041-1723&client=summon |
| thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=2041-1723&client=summon |