The novel bacteriocin BacYB1 produced by Leuconostoc mesenteroides YB1: From recent analytical characterization to biocontrol Verticillium dahliae and Agrobacterium tumefaciens

• Published in: Microbial pathogenesis Vol. 192; p. 106680
• Main Authors: Chakchouk-Mtibaa, Ahlem, Mechri, Sondes, Cheffi Azabou, Manel, Triki, Mohamed Ali, Smaoui, Slim, Mellouli, Lotfi
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01-07-2024
• Subjects: A. tumefaciens; Bacteriocin BacYB1; Biocontrol; Leuconostoc mesenteroides YB1 strain; MALDI imaging mass spectrometry; V. dahliae; MALDI imaging mass spectrometry; Bacteriocin BacYB1; V. dahliae; A. tumefaciens; Leuconostoc mesenteroides YB1 strain; Biocontrol
• ISSN: 0882-4010; 1096-1208; 1096-1208
• DOI: 10.1016/j.micpath.2024.106680

Biocontrol of phytopathogens involving the use of bioactive compounds produced by lactic acid bacteria (LAB), is a promising approach to manage many diseases in agriculture. In this study, a lactic acid bacterium designated YB1 was isolated from fermented olives and selected for its antagonistic activity against Verticillium dahliae (V. dahliae) and Agrobacterium tumefaciens (A. tumefaciens). Based on the 16S rRNA gene nucleotide sequence analysis (1565 pb, accession number: OR714267), the new isolate YB1 bacterium was assigned as Leuconostoc mesenteroides YB1 (OR714267) strain. This bacterium produces an active peptide “bacteriocin” called BacYB1, which was purified in four steps. Matrix-assisted lasers desorption/ionization (MALDI) time-of-flight (TOF) mass spectrometry (MS) based approach was performed to identify and characterize BacYB1. The exact mass was 5470.75 Da, and the analysis of the N-terminal sequence (VTRASGASTPPGTASPFKTL) of BacYB1 revealed no significant similarity to currently available antimicrobial peptides. The BacYB1 displayed a bactericidal mode of action against A. tumefaciens. The potentiel role of BacYB1 to supress the growth of A. tumefaciens was confirmed by live-dead cells viability assay. In pot experiments, the biocontrol efficacy of BacYB1 against V. dahliae wilt on young olive trees was studied. The percentage of dead plants (PDP) and the final mean symptomes severity (FMS) of plants articifialy infected by V. dahliae and treated with the pre-purified peptide BacYB1 (preventive and curative treatments) were significantly inferior to untreated plants. Biochemical analysis of leaves of the plants has shown that polyophenols contents were highly detected in plants infected by V. dahliae and the highest contents of chlorophyl a, b and total chlorophyll were recorded in plants treated with the combination of BacYB1 with the biofertilisant Humivital. BacYB1 presents a promising alternative for the control of Verticillium wilt and crown gall diseases.
•YB1, a new LAB strain selected for its biocontrol of V. dahliae and A. tumefaciens.•YB1 was identified as Leuconostoc mesenteroides (OR714267) YB1 strain.•Purification and characterization of bacteriocin BacYB1 were established.•Bactericidal effect of BacYB1 against A. tumefaciens was demonstrated.•BacYB1 prevents the verticillium wilt disease of olive plants.