Light-stimulated increase of translatable mRNA for phosphoenolpyruvate carboxylase in leaves of maize

• Published in: The Journal of biological chemistry Vol. 256; no. 16; pp. 8252 - 8255
• Main Authors: Sims, T.L, Hague, D.R
• Format: Journal Article
• Language: English
• Published: United States American Society for Biochemistry and Molecular Biology 25-08-1981
• Subjects: Carboxy-Lyases - genetics; Light; Phosphoenolpyruvate Carboxylase - genetics; Plants - enzymology; Protein Biosynthesis; RNA, Messenger - genetics; Triticum - metabolism; Zea mays - enzymology
• ISSN: 0021-9258; 1083-351X
• DOI: 10.1016/S0021-9258(19)68831-9

Polyadenylated RNA has been isolated from maize leaves at various times during greening of etiolated seedlings and used to
prime the wheat germ cell-free translation system. Levels of translatable messenger RNA for phosphoenolpyruvate carboxylase
increase with the length of the illumination period. The pattern of the increase in translatable mRNA for the enzyme is similar
to that of the increase in phosphoenolpyruvate carboxylase protein previously observed in intact tissues. Phosphoenolpyruvate
carboxylase synthesized in vivo migrates as a doublet band on gradient sodium dodecyl sulfate-polyacrylamide gels. A similar
doublet has been seen occasionally with the products of cell-free translation.