Elaboration of the method for immunocytochemical studying cytokinin-binding protein CBP70 localization in maize seedling tissues

• Published in: Russian journal of plant physiology Vol. 55; no. 4; pp. 552 - 559
• Main Authors: Vasil'eva, V. S, Lushnikova, A. L, Pavlik, L. L, Moshkov, D. A, Brovko, F. A
• Format: Journal Article
• Language: English
• Published: Dordrecht Dordrecht : SP MAIK Nauka/Interperiodica 01-07-2008; SP MAIK Nauka/Interperiodica
• Subjects: Biomedical and Life Sciences; cytokinin receptors; immunocytochemistry; Life Sciences; Methods; monoclonal antibodies; Plant Physiology; Plant Sciences; Zea mays; immunocytochemistry; Zea mays; cytokinin receptors; monoclonal antibodies
• ISSN: 1021-4437; 1608-3407
• DOI: 10.1134/S1021443708040183

Some approaches for the elaboration of the method for immunocytochemical detection of labile protein on sections prepared from maize (Zea mays L.) seedling tissues are outlined. The effects of severe chemical reagents applied during tissue pretreatment (fixatives, inhibitors of endogenous enzymes, solutions blocking nonspecific antibody binding) on labile protein immunoreactivity in extracts were assessed in preliminary experiments. The procedure was elaborated with a triple control, which provided for the lowest background staining on plant tissue sections and the high level of antibody specific binding.