Hybrid Offspring of C57BL/6J Mice Exhibit Improved Properties for Neurobehavioral Research
Abstract C57BL/6 is the most commonly used mouse strain in neurobehavioral research, serving as a background for multiple transgenic lines. However, C57BL/6 exhibit behavioral and sensorimotor disadvantages that worsen with age. We bred FVB/NJ females and C57BL/6J males to generate first-generation...
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| Published in: | eNeuro Vol. 9; no. 4; p. ENEURO.0221-22.2022 |
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| Main Authors: | , , , , , , , , , , |
| Format: | Journal Article |
| Language: | English |
| Published: |
Society for Neuroscience
01-07-2022
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| Subjects: | |
| Online Access: | Get full text |
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| Summary: | Abstract
C57BL/6 is the most commonly used mouse strain in neurobehavioral research, serving as a background for multiple transgenic lines. However, C57BL/6 exhibit behavioral and sensorimotor disadvantages that worsen with age. We bred FVB/NJ females and C57BL/6J males to generate first-generation hybrid offspring (FVB/NJ x C57BL/6J)F1. The hybrid mice exhibit reduced anxiety-like behavior, improved learning, and enhanced long-term spatial memory. In contrast to both progenitors, hybrids maintain sensorimotor performance upon aging and exhibit improved long-term memory. The hybrids are larger than C57BL/6J, exhibiting enhanced running behavior on a linear track during freely-moving electrophysiological recordings. Hybrids exhibit typical rate and phase coding of space by CA1 pyramidal cells. Hybrids generated by crossing FVB/NJ females with transgenic males of a C57BL/6 background support optogenetic neuronal control in neocortex and hippocampus. The hybrid mice provide an improved model for neurobehavioral studies combining complex behavior, electrophysiology, and genetic tools readily available in C57BL/6 mice. |
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| Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Author contributions: L.B. and E.S. designed research; H.E.S., L.B., A.L., O.A.-V., T.K., L.Sp., S.So., R.G., S.Si., and E.S. performed research; L.B. and E.S. contributed unpublished reagents/analytic tools; H.E.S., L.B., A.L., and E.S. analyzed data; H.E.S., L.B., L.Sj., and E.S. wrote the paper. This work was supported by the United States-Israel Binational Science Foundation (BSF) Grant 2015577; the European Research Council Grant 679253; the Israel Science Foundation Grant 638/16; the Israel Science Foundation FIRST Program Grant 1871/17; the Rosetrees Trust Grant A1576; the Canadian Institutes of Health Research (CIHR), the International Development Research Centre (IDRC), the Israel Science Foundation (ISF), and the Azrieli Foundation Grant 2558/18; and the Zimin Institute. The authors declare no competing financial interests. H.E.S. and L.B. contributed equally to this work. |
| ISSN: | 2373-2822 2373-2822 |
| DOI: | 10.1523/ENEURO.0221-22.2022 |