Effects of Aging on Intrinsic Protein Disorder in Human Lenses and Zonules

• Published in: Cell biochemistry and biophysics Vol. 82; no. 4; pp. 3667 - 3679
• Main Authors: Antonietti, Michael, Kim, Colin K., Djulbegovic, Mak B., Gonzalez, David J. Taylor, Greenfield, Jason A., Uversky, Vladimir N., Gibbons, Allister G., Karp, Carol L.
• Format: Journal Article
• Language: English
• Published: New York Springer US 01-12-2024; Springer Nature B.V
• Subjects: Aging; Amino acid composition; Amino acids; Biochemistry; Bioinformatics; Biological and Medical Physics; Biomedical and Life Sciences; Biophysics; Biotechnology; Cataracts; Cell Biology; Chi-square test; Comparative analysis; Composition; Lenses; Life Sciences; Original Paper; Pharmaceuticals; Pharmacology/Toxicology; Protein composition; Proteins; Proteomes; Proteomics; Statistical analysis; Statistical tests; Variance analysis; Zonules; Lens; proteomics; Intrinsically disordered proteins; bioinformatics
• ISSN: 1085-9195; 1559-0283; 1559-0283
• DOI: 10.1007/s12013-024-01455-x

This study aims to compare the levels of intrinsic protein disorder within the human lens and zonule proteomes and investigate the role of aging as a potential influencing factor on disorder levels. A cross-sectional proteomic analysis was employed, utilizing a dataset of 1466 proteins derived from the lens and zonule proteomes previously published by Wang et al. and De Maria et al. Bioinformatics tools, including a composition profiler and a rapid intrinsic disorder analysis online tool, were used to conduct a comparative analysis of protein disorder. Statistical tests such as ANOVA, Tukey’s HSD, and chi-squared tests were applied to evaluate differences between groups. The study revealed distinct amino acid compositions for each proteome, showing a direct correlation between aging and increased protein disorder in the zonular proteomes, whereas the lens proteomes exhibited the opposite trend. Findings suggest that age-related changes in intrinsic protein disorder within the lens and zonule proteomes may be linked to structural transformations in these tissues. Understanding how protein disorder evolves with age could enhance knowledge of the molecular basis for age-related conditions such as cataracts and pseudoexfoliation, potentially leading to better therapeutic strategies.