Proliferation of tumor spheroids after shock-wave treatment

• Published in: Journal of cancer research and clinical oncology Vol. 120; no. 7; pp. 439 - 441
• Main Authors: NICOLAI, H, STEINBACH, P, KNUECHEL-CLARKE, R, GRIMM, D, ROESSLER, W, WIELAND, W. F, HOFSTAEDTER, F
• Format: Journal Article
• Language: English
• Published: Berlin Springer 01-07-1994
• Subjects: Biological and medical sciences; Cell Division; Cell Line; Humans; In Vitro Techniques; Male; Medical sciences; Neoplasms - pathology; Other treatments; Prostatic Neoplasms - pathology; Treatment. General aspects; Tumors; Ultrasonics; Urinary Bladder Neoplasms - pathology; Cell proliferation; Urinary system disease; Intercellular transport; Spheroid; Malignant tumor; Adhesion; In vitro; Shock wave; Three dimensional model; Cell line; Urinary bladder; Models; Prostate; Ultrasound; Tumor cell
• ISSN: 0171-5216; 1432-1335
• DOI: 10.1007/BF01240146

Multicellular tumor spheroids (MCTS) grown from the bladder cancer cell line RT112 and from the prostate cancer cell line PCA were exposed to 200 or 800 electromagnetically generated focused ultrasound shock waves. RT112 cells showed a distinct but transient decrease in proliferation whereas the effect of PCA cells was less pronounced. Flow-cytometric measurements of DNA content and Ki67 expression revealed no significant changes in the cell cycle distribution. The capacity of RT112 cells exposed to 800 shock waves to re-grow as MCTS was markedly decreased, indicating an alteration of intercellular adhesion.