Inhibition of methionine uptake by methotrexate in mouse leukemia L1210 cells

Methionine-auxotrophic L1210 cells were used to study the effect of methotrexate (MTX) on methionine uptake and metabolism. MTX was shown to inhibit amino acid transport systems and cause a decrease of methionine uptake into L1210 cells. Conversely, a nonmetabolizable amino acid analogue reduced MTX...

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Bibliographic Details
Published in:	Cancer chemotherapy and pharmacology Vol. 19; no. 1; pp. 21 - 24
Main Authors:	SCANLON, K. J, CASHMORE, A. R, KASHANI-SABET, M, PALLAI, M, DREYER, R. N, MOROSON, B. A, SAKETOS, M
Format:	Journal Article
Language:	English
Published:	Berlin Springer 01-01-1987
Subjects:	Animals Antineoplastic agents Biological and medical sciences Biological Transport, Active - drug effects Cell Line Depression, Chemical General aspects Leukemia L1210 - metabolism Leukemia L1210 - pathology Medical sciences Methionine - biosynthesis Methionine - metabolism Methotrexate - metabolism Methotrexate - pharmacology Mice Pharmacology. Drug treatments Serine - metabolism Antineoplastic agent Antimetabolic Aminoacid Methionine L1210-Leukemia Metabolism Uptake Tumor cell
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Summary:	Methionine-auxotrophic L1210 cells were used to study the effect of methotrexate (MTX) on methionine uptake and metabolism. MTX was shown to inhibit amino acid transport systems and cause a decrease of methionine uptake into L1210 cells. Conversely, a nonmetabolizable amino acid analogue reduced MTX uptake into L1210 cells. MTX also blocked the transfer of the beta carbon from serine into methionine. Therefore, methionine deprivation may be an additional mechanism of action for MTX in methionine-auxotrophic tumor cells.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0344-5704 1432-0843
DOI:	10.1007/BF00296249