Chemical characterization and biological effect of exopolysaccharides synthesized by Antarctic yeasts Cystobasidium ongulense AL101 and Leucosporidium yakuticum AL102 on murine innate immune cells

• Published in: World journal of microbiology & biotechnology Vol. 39; no. 2; p. 39
• Main Authors: Rusinova-Videva, Snezhana, Ognyanov, Manol, Georgiev, Yordan, Petrova, Ani, Dimitrova, Petya, Kambourova, Margarita
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Netherlands 01-02-2023; Springer Nature B.V
• Subjects: Applied Microbiology; Biochemical engineering; Biochemistry; Biological effects; Biomass; Biomedical and Life Sciences; Biotechnology; Carbohydrates; Cultivation; Environmental Engineering/Biotechnology; Exopolysaccharides; Galactose; Genetic analysis; Glucans; Heterogeneity; Immune system; Interferon; Leucosporidium; Life Sciences; Macrophages; Mannan; Mannose; Microbiology; Molecular weight; Natural killer cells; NMR; Nuclear magnetic resonance; Two dimensional analysis; Yeast; Yeasts; γ-Interferon; Interferon-gamma; Chemical composition; Antarctic yeasts; Exopolysaccharide; Macrophages; Chromatography
• ISSN: 0959-3993; 1573-0972
• DOI: 10.1007/s11274-022-03477-0

The current study aimed to investigate exopolysaccharides (EPSs) produced by two Antarctic yeasts isolated from soil and penguin feathers samples collected on Livingston Island (Antarctica). The strains were identified as belonging to the species
Leucosporidium yakuticum
(LY) and
Cystobasidium ongulense
(CO) based on molecular genetic analysis. The EPS production was investigated using submerged cultivation. Different chemical, chromatographic, and spectral analyses were employed to characterize EPSs. LY accumulated 5.5 g/L biomass and 4.0 g/L EPS after 120 h of cultivation, while CO synthesized 2.1 g/L EPS at the end of cultivation, and the biomass amount reached 5.5 g/L. LY-EPS was characterized by a higher total carbohydrate content (80%) and a lower protein content (18%) by comparison with CO-EPS (62%, 30%). The LY-EPS mainly consisted of mannose (90 mol%), whereas CO-EPS had also glucose, galactose, and small amounts of uronic acids (8–5 mol%). Spectral analyses (FT-IR and 1D, 2D NMR) revealed that LY-EPS comprised a typical
β
-(1 → 4)-mannan. Branched (hetero)mannan, together with
β
/
α
-glucans constituted the majority of CO-EPS. Unlike LY-EPS, which had a high percentage of high molecular weight populations, CO-EPS displayed a large quantity of lower molecular weight fractions and a higher degree of heterogeneity. LY-EPS (100 ng/mL) elevated significantly interferon gamma (IFN-γ) production in splenic murine macrophages and natural killer (NK) cells. The results indicated that newly identified EPSs might affect IFN-γ signaling and in turn, might enhance anti-infectious responses. The data obtained also revealed the potential of EPSs and yeasts for practical application in biochemical engineering and biotechnology.