Optimization, kinetics, and radioprotective potential of a heteropolysaccharide from L. rhamnosus RVPI

• Published in: Biotechnology and applied biochemistry Vol. 67; no. 3; pp. 442 - 455
• Main Authors: Umesh, Anushri, , Pradeepa, Manjunath, Anushree, Salian, Chithralekha, , Manasadeepika, Suvarna, Manu, Muddappa, Vidya Shimoga
• Format: Journal Article
• Language: English
• Published: United States Wiley Subscription Services, Inc 01-05-2020
• Subjects: Antioxidants; Bacteria; Bile; Biodegradation; biokinetics; Computer simulation; Confidence intervals; Dextrose; Fermentation; Hydrophobicity; Intestine; Kinetics; L. rhamnosus, mechanistic models; Optimization; Peptone; Peptones; pH effects; Probiotics; radioprotective activity; Response surface methodology; RSM; Structural integrity; Substrates; radioprotective activity; Biokinetics; L. rhamnosus, mechanistic models; RSM; probiotics
• ISSN: 0885-4513; 1470-8744
• DOI: 10.1002/bab.1855

The probiotic potential of Lactobacillus rhamnosus RVP1 isolated from Sardinella longiceps was investigated in vitro. The bacterium exhibited highest tolerance at low pH and high bile salt concentration and demonstrated good antioxidant activity, hydrophobicity, and inhibited both Gram‐negative and Gram‐positive indicator bacteria. To aid in process design and to unravel the fermentation kinetics, response surface methodology was devised to optimize the EPS production from L. rhamnosus and mechanistic models were developed to describe the fermentation kinetics. The optimum pH and dextrose and peptone concentrations for EPS production were 7.07 and 19.995 and 23.4 g/L, respectively, with a predicted yield of 724 mg/L. The actual yield under these conditions was 708 ± 29 mg/L that was within the 95% confidence interval. The simulated mechanistic model fit the experimental values with a high degree of correlation with R2 = 0.99, 0.96, and 0.97 for the logistic growth, substrate consumption, and EPS production and degradation curves, respectively. The kinetic constants µmax = 0.29/H, Xmax = 3.44 g/L, kf = 348 mg of EPS/g of dry biomass and kd = 0.53/H were derived from the model. The EPS administration improved the survival of irradiated mice by 50%, proving its radioprotective potential and showed positive effects on structural integrity of intestinal tissue.
Preliminary investigation on the production kinetics and radioprotective application of EPS from L. Rhamnosus.