Floral development stage and its implications for the reproductive success of Passiflora L

• Published in: Scientia horticulturae Vol. 238; pp. 333 - 342
• Main Authors: Soares, Taliane Leila, Jesus, Onildo Nunes de, Souza, Everton Hilo de, Oliveira, Eder Jorge de
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 19-08-2018
• Subjects: Fruiting; Passifloraceae; Pollen viability; Pollination; Stigma receptivity; IPG; FDA; Fruiting; Passifloraceae; Stigma receptivity; PGV; Pollen viability; Pollination
• ISSN: 0304-4238; 1879-1018
• DOI: 10.1016/j.scienta.2018.04.034

•We examined the floral and reproductive biology of several Passiflora species in different floral development stages.•FDA and Alexander’s solution overestimated the pollen viability in comparison with the in vitro pollen germination test.•Anthesis stage provided the highest percentages of pollen viability and stigma receptivity in different Passiflora species.•P. cincinnata, P. edulis and P. suberosa presented stigma receptivity in the three floral development stages.•Inhibited pollen tubes and deposited callose was observed in tubes in pre-anthesis stage.
Most Passiflora species present self-incompatibility and thus depend on cross-pollination for fruit production. Therefore, the objective of this work was to evaluate the floral and reproductive biology of 17 Passiflora species, through pollen viability, stigma receptivity, fruit and seed set formation, as well as the pollen-pistil interaction in three floral development stages (pre-anthesis, anthesis and post-anthesis), seeking to maximize the efficiency of controlled pollinations. Pollen viability was estimated by in vitro pollen germination, fluorochromatic reaction and Alexander’s solution, as well as fruiting and pollen-pistil interaction. The stigma receptivity was determined in alpha-naphthyl acetate. The highest percentages of pollen viability, stigma receptivity, fruit and seed set were observed during floral anthesis for all Passiflora species studied, while the lowest values were observed in pre-anthesis. The species P. rubra L. presented the highest percentages of in vitro pollen germination, viability with FDA, stigma receptivity, pollen/pistil compatibility, and high fruit set in anthesis. In contrast, the lowest values of these traits were observed in P. galbana L. The results obtained can support the selection of genetic material and allow inferences about the best pollen collection moment and the peak stigma receptivity to maximize the chances of pollen germination and consequently increase the efficiency of controlled pollinations for both agronomic and ornamental purposes.