Hydrogen peroxide promotes the tolerance of soybeans to waterlogging

• Published in: Scientia horticulturae Vol. 232; pp. 40 - 45
• Main Authors: Andrade, Cinthia Aparecida, Souza, Kamila Rezende Dázio de, Santos, Meline de Oliveira, Silva, Dayane Meireles da, Alves, Jose Donizeti
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 17-02-2018
• Subjects: Antioxidants; Flooding; Gas exchange; Glycine max; Antioxidants; Gas exchange; Flooding; Glycine max
• ISSN: 0304-4238; 1879-1018
• DOI: 10.1016/j.scienta.2017.12.048

•Soybean seeds were pretreated with H2O2 and seedlings were waterlogged.•H2O2 pretreatment improved soybean tolerance to waterlogging.•H2O2 pretreatment improved antioxidant system activity and reduced ROS generation.
We investigated the role of pretreatment of seeds with hydrogen peroxide (H2O2) in promoting the tolerance of seedlings of Glycine max to waterlogging. Soybean seeds were pretreated with a solution of H2O2 70 mM or water for 24 h under aeration, and then were placed in plastic bags containing substrate. At 12 days after sowing, the seedlings were subjected to waterlogging for 32 days. There were three evaluations: 0, 16 days and 32 days of waterlogging. Biomass accumulation, root volume, stem diameter, gas exchanges, activity of antioxidant system and cell membrane damages were evaluated. The pretreatment of seeds with H2O2 generated seedlings with elevated activity of antioxidant enzymes, low H2O2 and O2−. content, and low cell membrane damages. Hydrogen peroxide pretreatment also resulted in increased content of photosynthetic pigments and net photosynthetic rate, high biomass accumulation, root volume and stem diameter. H2O2 pretreatment of soybeans seeds generated seedlings more tolerant to waterlogging. There is probably a stress memory involved in the process of induction of tolerance in soybeans mediated by hydrogen peroxide.