Sprouting of afferent and efferent inputs to pelvic organs after spinal cord injury

• Published in: Journal of neuropathology and experimental neurology Vol. 83; no. 1; pp. 20 - 29
• Main Authors: Zhao, Shunyi, Tang, Chuanxi, Weinberger, Jeremy, Gao, Dianshuai, Hou, Shaoping
• Format: Journal Article
• Language: English
• Published: England Oxford University Press 22-12-2023
• Subjects: Bladder; Morphology; Spinal cord injuries; Calcitonin gene-related peptide; Tyrosine hydroxylase; Sprout; Distribution; Peripheral system
• DOI: 10.1093/jnen/nlad108
• EISSN: 1554-6578
• ISSN: 0022-3069

Abstract
Neural plasticity occurs within the central and peripheral nervous systems after spinal cord injury (SCI). Although central alterations have extensively been studied, it is largely unknown whether afferent and efferent fibers in pelvic viscera undergo similar morphological changes. Using a rat spinal cord transection model, we conducted immunohistochemistry to investigate afferent and efferent innervations to the kidney, colon, and bladder. Approximately 3–4 weeks after injury, immunostaining demonstrated that tyrosine hydroxylase (TH)-labeled postganglionic sympathetic fibers and calcitonin gene-related peptide (CGRP)-immunoreactive sensory terminals sprout in the renal pelvis and colon. Morphologically, sprouted afferent or efferent projections showed a disorganized structure. In the bladder, however, denser CGRP-positive primary sensory fibers emerged in rats with SCI, whereas TH-positive sympathetic efferent fibers did not change. Numerous CGRP-positive afferents were observed in the muscle layer and the lamina propria of the bladder following SCI. TH-positive efferent inputs displayed hypertrophy with large diameters, but their innervation patterns were sustained. Collectively, afferent or efferent inputs sprout widely in the pelvic organs after SCI, which may be one of the morphological bases underlying functional adaptation or maladaptation.