Effects of Eugenol and Dehydrodieugenol B from Nectandra leucantha against Lipopolysaccharide (LPS)-Induced Experimental Acute Lung Inflammation

• Published in: Journal of natural products (Washington, D.C.) Vol. 84; no. 8; pp. 2282 - 2294
• Main Authors: Bittencourt-Mernak, Márcia I, Pinheiro, Nathalia M, da Silva, Rafael C, Ponci, Vitor, Banzato, Rosana, Pinheiro, Aruanã J M C R, Olivo, Clarice R, Tibério, Iolanda F L C, Lima Neto, Lídio G, Santana, Fernanda P R, Lago, João H G, Prado, Carla M
• Format: Journal Article
• Language: English
• Published: United States 27-08-2021
• Subjects: Acute Lung Injury - chemically induced; Acute Lung Injury - drug therapy; Animals; Anisoles - pharmacology; Brazil; Eugenol - pharmacology; Lauraceae - chemistry; Lipopolysaccharides; Male; Mice; Mice, Inbred BALB C; Phytochemicals - pharmacology; Plant Leaves - chemistry; Pneumonia - chemically induced; Pneumonia - drug therapy; RAW 264.7 Cells; Brazil
• ISSN: 0163-3864; 1520-6025
• DOI: 10.1021/acs.jnatprod.1c00386

Acute lung injury (ALI) is an important public health problem. The present work investigated whether dehydrodieugenol B treatment, a compound isolated from Brazilian plant
(Lauraceae), modulates experimental ALI and compared the observed effects to eugenol. Effects of dehydrodieugenol B
in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells were evaluated. The lung and systemic inflammatory profile, lung function, and possible mechanisms involved in BALB/C male mice (6-8 weeks) with ALI induced by LPS instillation (5 mg/kg) was assayed. Dehydrodieugenol B did not affect the cell viability and inhibited the increase in NO release and IL-1β and IL-6 gene expression induced by LPS.
, both compounds reduced lung edema, inflammatory cells, and the IL-6 and IL-1 β levels in bronchoalveolar lavage fluid, as well as reduced inflammatory cell infiltration and those positive to iNOS, MMP-9, and TIMP-1, and reduced the collagen content and the 8-isoprostane expression in lung tissue. Eugenol and dehydrodieugenol B also inhibited the phosphorylation of Jc-Jun-NH2 terminal Kinase (JNK), a signaling protein involved in the MAPKinase pathway. There was no effect of these compounds in lung function. Therefore, eugenol and dehydrodieugenol B ameliorates several features of experimental ALI and could be considered as a pharmacological tool to ameliorate acute lung inflammation.