What place for polyacrylamide in proteomics?

• Published in: Trends in Biotechnology Vol. 19; no. 10 Suppl; pp. 3 - 9
• Main Authors: Herbert, Ben R, Harry, Jenny L, Packer, Nicolle H, Gooley, Andrew A, Pedersen, Susanne K, Williams, Keith L
• Format: Book Review Journal Article
• Language: English
• Published: England Elsevier Ltd 01-10-2001
• Subjects: 2D gels; Amino Acid Sequence; Electrophoresis, Gel, Two-Dimensional - methods; Electrophoresis, Gel, Two-Dimensional - trends; fractionation; membrane proteins; Membrane Proteins - analysis; Membrane Proteins - chemistry; Molecular Biology - methods; Molecular Biology - trends; Molecular Sequence Data; polyacrylamide; protein chip; Protein Isoforms; Proteins - analysis; Proteins - chemistry; proteomics; sample preparation; seperation technolgy; spliced variants; fractionation; Biotechnology; spliced variants; membrane proteins; Techniques & Methods; 2D gels; protein chip; Drug Discovery; sample preparation; seperation technolgy
• ISSN: 0167-7799; 1879-3096
• DOI: 10.1016/S0167-7799(01)00002-6

Two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) continues to deliver high quality protein resolution and dynamic range for the proteomics researcher. To remain as the preferred method for protein separation and characterization, several key steps need to be implemented to ensure quality sample preparation and speed of analysis. Here, we describe the progress made towards establishing 2D-PAGE as the optimal separation tool for proteomics research.