Protection of a protein against irradiation-induced degradation by additives in the solid state

• Published in: Radiation physics and chemistry (Oxford, England : 1993) Vol. 66; no. 3; pp. 237 - 245
• Main Authors: Shalaev, E, Reddy, R, Kimball, R.N, Weinschenk, M.F, Guinn, M, Margulis, L
• Format: Journal Article
• Language: English
• Published: Elsevier Ltd 01-03-2003
• Subjects: Additives; ESR; Free radicals; Irradiation; Protein; Solid state; Stabilization; Sterilization; Solid state; Additives; Free radicals; ESR; Stabilization; Sterilization; Irradiation; Protein
• ISSN: 0969-806X; 1879-0895
• DOI: 10.1016/S0969-806X(02)00287-6

The impact of ionizing radiation on a globular protein (porcine somatotropin, pST) in the solid state was studied using rate of dissolution, high-performance liquid chromatography, and Electron spin resonance (ESR) in the presence of different additives.
o-Vanillin stabilized pST against irradiation-induced degradation whereas effects of trolox and isopropyl alcohol were less significant. Stabilization effect of
o-vanillin has been related to the energy transfer from pST molecules to the additive which was facilitated by formation of covalent bonds between
o-vanillin and pST molecules. Anticorrelation between the level of free radicals and chemical degradation (i.e. degradation increased with decrease in a free radical level) was observed in the presence of
o-vanillin.