Birc3 and Tip1 are upregulated in renal ischemia reperfusion injury

• Published in: Gene Vol. 876; p. 147492
• Main Authors: Wang, Sixu, Zhao, Meishan, Zhang, Xiaofei, Su, Ming, Tian, Ye, Qiu, Wei
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 05-08-2023
• Subjects: Animals; Apoptosis; Apoptosis - genetics; Baculoviral IAP Repeat-Containing 3 Protein - genetics; Birc3; Epithelial Cells - metabolism; Intracellular Signaling Peptides and Proteins - genetics; Ischemia reperfusion injury; Kidney - metabolism; Mice; Renal transplantation; Reperfusion Injury - metabolism; Tip1; Up-Regulation; DGF; IHC; TECs; Birc3; EAD; I/R; Cr; Tip1; FCM; DEGs; TUNEL; Ischemia reperfusion injury; OGD/R; Renal transplantation; BUN; Apoptosis
• ISSN: 0378-1119; 1879-0038
• DOI: 10.1016/j.gene.2023.147492

•Birc3 and Tip1 were upregulated in renal ischemia reperfusion (I/R) mice model.•Birc3 was upregulated in human tubular epithelial cells after I/R, while Tip1 was downregulated.•Birc3 may play a protective role in I/R by resisting apoptosis of renal tubular epithelial cells.
Identification of ischemia–reperfusion injury (I/R)-associated genes is essential for exploring I/R novel mechanisms. Previously, we screened differentially expressed genes in renal I/R mouse models and found that Tax1 binding protein 3 (Tip1) and baculoviral IAP repeat containing 3 (Birc3) are two upregulated genes in I/R. In the present study, we analyzed the expression of Tip1 and Birc3 in I/R models. We found that the expression of Tip1 and Birc3 was upregulated in I/R-treated mice, whereas Tip1 was downregulated and Birc3 was upregulated in oxygen-glucose deprivation/reoxygenation (OGD/R) in vitro models. By inhibiting Birc3 with AT-406 in I/R-treated mice, we observed that the serum creatinine or blood urea nitrogen did not vary. However, inhibition of Birc3 enhanced apoptosis of kidney tissues induced by I/R treatment. Consistently, we found that inhibition of Birc3 also increased the apoptosis rate in tubular epithelial cells induced by OGD/R. These data demonstrated that Tip1 and Birc3 were upregulated in I/R injury. The upregulation of Birc3 may protect against renal I/R injury.