Preparation and characterization of monoclonal antibodies to substance P

• Published in: Hybridoma Vol. 10; no. 6; p. 685
• Main Authors: Frickey, P H, Yanni, J M, Appell, K
• Format: Journal Article
• Language: English
• Published: United States 01-12-1991
• Subjects: Animals; Antibodies, Monoclonal - immunology; Antibodies, Monoclonal - isolation & purification; Antibodies, Monoclonal - metabolism; Binding Sites; Binding, Competitive; Guinea Pigs; Hybridomas - immunology; Ileum - drug effects; Immunoglobulin G - immunology; Immunoglobulin G - isolation & purification; Immunoglobulin G - metabolism; Immunoglobulin M - immunology; Immunoglobulin M - isolation & purification; Immunoglobulin M - metabolism; Mice; Muscle Contraction - drug effects; Muscle, Smooth - drug effects; Protein Binding; Receptors, Neurokinin-2; Receptors, Neurotransmitter - metabolism; Substance P - antagonists & inhibitors; Substance P - immunology; Substance P - pharmacology
• ISSN: 0272-457X
• DOI: 10.1089/hyb.1991.10.685

A series of mouse hybridomas that produced monoclonal antibodies reactive with Substance P was prepared in an attempt to find a surrogate NK-1 receptor. When the binding profile of one particular antibody, SubP14.36.1, was compared in a rank order plot with NK-1 receptors against various agonists and antagonists of Substance P, a high correlation was found between these two binding structures. In addition, this monoclonal antibody inhibited a functional assay for Substance P. This result indicated that the pharmacological effects of this peptide can be blocked by an antibody (Ab).