Genetic variability of the tropical grasshopper Poecilocerus bufonius in Saudi Arabia

• Published in: Archiv für Phytopathologie und Pflanzenschutz Vol. 44; no. 17; pp. 1736 - 1744
• Main Authors: Elsayed, G, Amer, Sayed A.M, Sayed, Samy M.H
• Format: Journal Article
• Language: English
• Published: Abingdon Taylor & Francis 01-10-2011; Taylor & Francis Ltd
• Subjects: acid phosphatase; alcohol dehydrogenase; alleles; electrophoresis; enzymes; genetic variability; genetic variation; grasshoppers; isozymes; loci; mountains; peroxidase; polyacrylamide gel electrophoresis; protein bands; Saudi Arabia; tropical grasshopper; Saudi Arabia
• ISSN: 1477-2906; 0323-5408; 1477-2906
• DOI: 10.1080/03235408.2010.544468

Electrophoresis for SDS-proteins and isoenzymes was applied to investigate the genetic variability within the tropical grasshopper Poecilocerus bufonius, which inhabits Saudi Arabia. Samples were selected from different localities that are encompassing the Sarawat Mountains in the west and An-Nafud desert in the north and middle. A range of 2 to 14 protein bands were recorded in the studied samples as measured by SDS-polyacrylamide gel electrophoresis, from which only two were common. The maximum number of protein bands has been recorded in the samples from the west and the minimum number has been recorded in the samples from the north and middle. Six arbitrary chosen enzymes were examined by native-polyacrylamide gel electrophoresis. They were peroxidase (Px), aldehyde oxidase (Ao), acid phosphatase (Acph), alcohol dehydrogenase (Adh), á and â ePsterase (Est). Fourteen presumptive gene loci and 26 polymorphic alleles have been recorded with the highest number of alleles in Taif and minimum number of alleles in Qassim. Adh, Px and Acph have not been recorded in samples from the localities of An-Nafud desert. The samples from Taif (Sarawat Mountains) were more genetically variable than the samples from other localities. Most of alleles were monomeric but only the Px-1 showed trimeric alleles in samples from Taif.