Hemoglobin Regeneration Efficiency and Relative Iron Bioavailability of Four Elemental Iron Powders in Rats

• Published in: Nutrients Vol. 16; no. 14; p. 2258
• Main Authors: Swain, James H, Nemeth, Ryan C, Bethi, Anshul R, Jang, Caroline J, Zheng, Eva L
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 13-07-2024
• Subjects: absorption; Anemia, Iron-Deficiency - drug therapy; Animals; Bioavailability; Biological Availability; Diet; Efficiency; elemental iron powder; Ferrous Compounds - administration & dosage; Food; Food, Fortified; fortification; Hemoglobin; hemoglobin regeneration efficiency; Hemoglobins - metabolism; Hydrochloric acid; Iron; Iron - blood; Iron, Dietary - administration & dosage; Iron, Dietary - pharmacokinetics; Male; Powders; Rats; Rats, Sprague-Dawley; relative bioavailability; United States > US; hemoglobin regeneration efficiency; relative bioavailability; elemental iron powder; absorption; fortification
• ISSN: 2072-6643; 2072-6643
• DOI: 10.3390/nu16142258

Effective food fortification strategies using elemental iron powders (EIPs) are needed to combat iron deficiency anemia. The purpose of this study was to determine hemoglobin regeneration efficiency (HRE) and relative iron bioavailability (RBV) of four food-grade EIPs (El-Lyte (EL), Hi-Sol (HS), H-325 (H3), and A-131 (A1)) by treating anemic rats with 14 d iron repletion diets (uncooked and cooked), fortified with a 12, 24, or 36 mg iron/kg diet of the EIPs, ferrous sulfate monohydrate (FS, FeSO
•H
O), or no added iron (control), n = 9-12/group. The ability of EL and HS to maintain hemoglobin for 6 weeks on the 6 mg iron/kg diet was also studied. The dissolution rate of iron from the EIPs was measured in hydrochloric acid at pH 1.0. Compared to FS, the EL, HS, and A1 EIPs had >50% overall RBV, with the following order: HS > A1 > EL > H3 (
≤ 0.05); the effect of cooking was not significant (
> 0.05). Dissolution testing revealed that the mean RBV of the EIPs was positively associated with the percentage of iron solubility. In the 6-week maintenance study, EL and HS maintained hemoglobin as well as FS. Overall, the findings show that at the concentrations of iron tested, these EIPs are effective fortification agents to replenish hemoglobin and correct iron deficiency anemia.