Aqueous extract of pummelo pulp (Citrus maxima) improves the biochemical profile and reduces the inflammation process in Wistar rats with non-alcoholic fatty liver disease

• Published in: Food and chemical toxicology Vol. 178; p. 113933
• Main Authors: Tejada Nunes, Vinícius, Gonçalves, Itamar Luís, Martinez Oliveira, Patricia, Lima Feksa, Denise, Muller de Moura Sarmento, Sílvia, Erminda Schreiner, Gênifer, Klock, Clóvis, Casanova Petry, Charline, da Costa Escobar Piccoli, Jacqueline, Manfredini, Vanusa, Casagrande Denardin, Cristiane
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01-08-2023
• Subjects: Animals; Biochemical parameters; Citrus; Diet, High-Fat - adverse effects; Inflammation - metabolism; Inflammatory markers; Liver; Male; Non-alcoholic fatty liver disease; Non-alcoholic Fatty Liver Disease - metabolism; Oxidative Stress; Plant Extracts - pharmacology; Plant Extracts - therapeutic use; Pummelo; Rats; Rats, Sprague-Dawley; Rats, Wistar; Oxidative stress; Inflammatory markers; Biochemical parameters; Non-alcoholic fatty liver disease; Pummelo
• ISSN: 0278-6915; 1873-6351
• DOI: 10.1016/j.fct.2023.113933

This study investigated the effect of pummelo extract (Citrus maxima) on biochemical, inflammatory, antioxidant and histological changes in NAFLD rats. Forty male Wistar rats divided into four groups were used: (1) control group; (2) fructose associated with high-fat diet - DHF; (3) normal diet + pummelo extract (50 mg/kg); and (4) FHD + pummelo extract. This was administered at dose of 50 mg/kg of the animal's weight, by gavage, for 45 days. Significant improvement in lipid profile, liver and kidney function, inflammation, oxidative stress markers was identified in group 4 compared to group 2. Regarding TNF-α and IL-1β, group 2 showed higher values (respectively 142, 5 ± 0.7 and 560.5 ± 2.7 pg/mg protein) compared to group 4 (respectively 91.4 ± 0.9 and 402.1.4 ± 0.9 pg/mg protein), p < 0.05. Significant increases were found in SOD and CAT activities, respectively 0.10 ± 0.06 and 8.62 ± 1.67 U/mg protein for group 2 and respectively 0.28 ± 0.08 and 21.52 ± 2.28 U/mg of protein for group 4. Decreases in triglycerides, hepatic cholesterol and fat droplets in hepatic tissue were observed in group 4 compared to group 2. Results highlight that pummelo extract may be useful for prevent the development of NAFLD.
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