Cadmium Resistance in Pseudomonas putida: Growth and Uptake of Cadmium

• Published in: Journal of general microbiology Vol. 131; no. 10; pp. 2539 - 2544
• Main Authors: HIGHAM, DENISE P, SADLER, PETER J, SCAWEN, MICHAEL D
• Format: Journal Article
• Language: English
• Published: London Soc General Microbiol 01-10-1985; New York, NY Cambridge University Press
• Subjects: Action of physical and chemical agents on bacteria; Bacteriology; Biological and medical sciences; Fundamental and applied biological sciences. Psychology; Microbiology; Pseudomonas putida; Pseudomonadales; Resistance; Cadmium; Growth; Microorganism culture; Bacteria; Pseudomonadaceae; Pseudomonas putida; Accumulation; Release; Adaptation
• ISSN: 0022-1287; 1350-0872; 1465-2080
• DOI: 10.1099/00221287-131-10-2539

1 Department of Chemistry, Birkbeck College, University of London, Malet Street, London WC1E 7HX, UK
2 Microbial Technology Laboratory, Centre for Applied Microbiology and Research, Porton Down, Salisbury, Wiltshire SP4 OJG, UK
ABSTRACT
Summary: A strain of Pseudomonas putida resistant to low concentrations of cadmium (0.25 mM-Cd 2+ ) adapted to growth in the presence of 3 mM-Cd 2+ in a chemically defined medium. This increased resistance was rapidly lost if Cd 2+ was omitted from the growth medium. P. putida concentrated 109 Cd 2+ actively. Mechanisms appeared to exist for expelling cadmium from cells during the lag and exponential phases and for continued growth in the presence of high intracellular Cd 2+ concentrations. Cd 2+ -resistant cells adapted so as to control both the extent and rate of Cd 2+ uptake when compared to control cells.