Nine polymorphic markers characterized by polymerase chain reaction techniques form two linkage groups on rat chromosome 8

• Published in: Cytogenetics and cell genetics Vol. 66; no. 4; p. 283
• Main Authors: Mathern, P, Goldmuntz, E A, Du, Y, Zha, H, Cash, J M, Crofford, L J, Wilder, R L, Remmers, E F
• Format: Journal Article
• Language: English
• Published: Switzerland 1994
• Subjects: Alleles; Animals; Antigens, Surface - genetics; Base Sequence; Chromosome Mapping; Crosses, Genetic; DNA Primers; Genetic Linkage; Genetic Markers; Matrix Metalloproteinase 7; Membrane Glycoproteins - genetics; Metalloendopeptidases - genetics; Molecular Sequence Data; Peptide Hydrolases - genetics; Polymerase Chain Reaction - methods; Polymorphism, Genetic; Rats; Rats, Inbred Strains - genetics; Repetitive Sequences, Nucleic Acid; Retinol-Binding Proteins - genetics; Retinol-Binding Proteins, Cellular; Species Specificity; Thy-1 Antigens; Tropoelastin - genetics
• ISSN: 0301-0171
• DOI: 10.1159/000133713

Five genes and four anonymous polymorphic markers, forming two linkage groups, were mapped in F2 intercross progeny of F344/N x LEW/N rats using polymerase chain reaction (PCR) techniques. Both linkage groups were assigned to rat chromosome 8 because they contained genetic loci previously mapped to this chromosome. The first group was comprised of markers for three anonymous loci and two gene loci, thymus cell antigen-1 (Thy1) and tropoelastin (Eln). The second group was comprised of markers for one anonymous locus and three gene loci, cellular retinol binding protein II (Rbp2), matrin F/G (Matr1), and acyl-peptide hydrolase (Apeh). Seven markers (identified by simple sequence repeat associated length polymorphisms) were characterized in an additional 13 inbred rat strains (ACI/N, BN/SsN, BUF/N, LER/N, LOU/MN, MNR/N, MR/N, SHR/N, SR/Jr, SS/Jr, WBB1/N, WBB2/N, and WKY/N). Two to six alleles were detected for each marker. The reported markers should facilitate genetic mapping and monitoring of inbred rat strains.