Spin Filter Perfusion System for High Density Cell Culture: Production of Recombinant Urinary Type Plasminogen Activator in CHO Cells

We have used a 20 liter stirred tank fermentor, equipped with a 127 mesh ethylene-tetrafluoroethylene rotating screen for cell recycle, for the continuous production of recombinant single chain urokinase-type plasminogen activator (rscu-PA) from Chinese hamster ovary (CHO) cells. Viable cell densiti...

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Bibliographic Details
Published in:	Bio/technology (New York, N.Y. 1983) Vol. 8; no. 1; pp. 54 - 58
Main Authors:	Avgerinos, George C, Drapeau, Denis, Socolow, Jeff S, Mao, Jen-i, Hsiao, Kathy, Broeze, Robert J
Format:	Journal Article
Language:	English
Published:	New York, NY Nature Publications 01-01-1990
Subjects:	Animals Biological and medical sciences Biotechnology Biotechnology - methods Cell Count Cell Division Cell Line Cell Survival Cloning, Molecular Cricetinae Electrophoresis, Polyacrylamide Gel Fundamental and applied biological sciences. Psychology Gene Expression Health. Pharmaceutical industry Industrial applications and implications. Economical aspects Other active biomolecules Plasminogen Activators - biosynthesis Plasminogen Activators - genetics Plasminogen Activators - isolation & purification Production of active biomolecules Recombinant Proteins - biosynthesis Recombinant Proteins - genetics Transfection - genetics Urokinase-Type Plasminogen Activator - biosynthesis Urokinase-Type Plasminogen Activator - genetics Urokinase-Type Plasminogen Activator - isolation & purification Cell culture Enzyme Plasminogen activator Mixed tank reactor CHO cell line Optimization Cell density Continuous process Bioreactor Production Recombinant protein Recycling Viability
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