Comparison of the standard AGID test and competitive ELISA for detecting bluetongue virus antibodies in camels in Gujarat, India

• Published in: Tropical animal health and production Vol. 35; no. 2; pp. 99 - 104
• Main Authors: Chandel, B.S, Chauhan, H.C, Kher, H.N
• Format: Journal Article
• Language: English
• Published: United States Springer Nature B.V 01-04-2003
• Subjects: agar gel immunodiffusion test; animal diseases; Animals; Antibodies, Viral - immunology; antibody formation; blood serum; bluetongue; Bluetongue - diagnosis; Bluetongue - immunology; Bluetongue virus; Bluetongue virus - immunology; breed differences; Camelus - immunology; Camelus - virology; disease prevalence; dromedaries; enzyme-linked immunosorbent assay; Enzyme-Linked Immunosorbent Assay - veterinary; Immunodiffusion - veterinary; immunodiffusion tests; India; Kutchhi (dromedary breed); lameness; Marwari (dromedary breed); seroconversion; seroprevalence; Species Specificity; stiffness; trypanosomiasis; India
• ISSN: 0049-4747; 1573-7438
• DOI: 10.1023/A:1022896117122

The performance of the standard agar gel immunodiffusion (AGID) test and the competitive enzyme-linked immunosorbent assay (cELISA) for the detection of serum antibody against bluetongue virus (BTV) in clinically healthy and diseased camels in Gujarat state were compared. Out of 176 sera tested, 22 (12.5%) and 34 (19.3%) were positive for group-specific bluetongue antibodies by AGID and cELISA, respectively. Maximum seropositivities of 18.0% by AGID and 25.8% by cELISA were recorded in the Kutchhi breed, and of 6.9% and 12.6%, respectively, in the Marwari breed. The seroprevalence detected by AGID and cELISA in clinically healthy and diseased camels did not differ significantly with regard to bluetongue disease in these breeds.