Automated Assessment of the Area of Infiltration by CD8+ Cells in Gastric Carcinoma and Areas of Normal Mucosa as a Significant Prognostic Factor

• Published in: Bulletin of experimental biology and medicine Vol. 175; no. 4; pp. 508 - 512
• Main Authors: Danilova, N. V., Mikhailov, I. A., Khomyakov, V. M., Chaika, A. V., Polushkina, T. V., Sotnikova, T. N., Oleinikova, N. A., Mal’kov, P. G.
• Format: Journal Article
• Language: English
• Published: New York Springer US 01-08-2023; Springer Nature B.V
• Subjects: Automation; Biomedical and Life Sciences; Biomedicine; CD8 antigen; Cell Biology; Confidence intervals; Gastric cancer; Infiltration; Internal Medicine; Invasiveness; Laboratory Medicine; Medical prognosis; Metastases; Metastasis; Morphology; Morphology and Pathomorphology; Morphometry; Mucosa; Pathology; Software; Statistical analysis; Survival analysis; Tumors; T lymphocytes; morphometry; immune microenvironment; gastric cancer; prognostic markers
• ISSN: 0007-4888; 1573-8221
• DOI: 10.1007/s10517-023-05896-x

In 139 patients with verified gastric cancer, the infiltration of the postoperative material with CD8
+
cells was analyzed. Automated morphometric analysis of immunostained slides was performed separately in different specimen sites (tumor center, invasive edge, and peritumoral mucosa). The mean area of infiltrating CD8
+
cells in the tumor center and in the invasive edge was not predictive, while in the peritumoral mucosa it provided a new negative predictive factor (hazard ratio 2.10; confidence interval 0.87-4.92, Cox regression) reliably associated with the TNM stage (hazard ratio 1.91; confidence interval 0.91-4.61, Cox regression).