Antihypertensive effects of the Vitis vinifera grape skin (ACH09) extract consumption elicited by functional improvement of P1 (A1) and P2 (P2X1) purinergic receptors in diabetic and hypertensive rats

• Published in: PharmaNutrition Vol. 8; p. 100146
• Main Authors: Bomfim, Guilherme Henrique Souza, Musial, Diego Castro, Miranda-Ferreira, Regiane, Nascimento, Sthefani Rodrigues, Jurkiewicz, Aron, Jurkiewicz, Neide Hyppolito, de Moura, Roberto Soares
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 01-06-2019
• Subjects: Diabetes; Grape skin extract; Hypertension; Purinergic receptors; Sympathetic neurotransmission; NO; WKY; ADO; PHE; EAm; KHN; Sympathetic neurotransmission; AUCs; ACH09; SHR; T0; τa; SBP; Hypertension; ARs; VSMCs; ISO; Purinergic receptors; τi; pIC50; NIE; RA; NAD; T30; Grape skin extract; LA; Diabetes; OGTT; ATP
• ISSN: 2213-4344; 2213-4344
• DOI: 10.1016/j.phanu.2019.100146

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Cross-talk between hypertension and diabetes associated with red wine consumption triggering cardioprotective and antihypertensive effects have been well established; nevertheless, the role of isolated total polyphenols from extracts of Vitis vinifera grape skin (ACH09) and the possible correlation with cardiovascular functionality mediated by purinergic receptors remain poorly understood. We evaluate how ACH09 extract causes an antihypertensive effect by modulation of purinergic receptors. Male Wistar Kyoto (WKY), streptozotocin-induced diabetic WKY and SHR (spontaneously hypertensive) rats were subjected to intake of ACH09 extract 200 mg/kg/v.o. for 21 days. Systolic blood pressure (SBP), blood glucose levels were measured; cardiovascular functionality of sympathetic neurotransmission, represented by purinergic (P1-A1/P2-P2X1) and adrenergic receptors (α1/β1), was evaluated by isometric contraction. Compared to their respective controls, we found that ACH09 extract exposure decreased SBP in both groups, primarily by functional improvement of the following receptors: (1) P1 purinergic (A1), augmenting vascular endothelium-dependent relaxation; (2) P2 purinergic (P2X1), decreasing vasoconstriction; and (3) P1 purinergic (A1), increasing atrial negative inotropism. In conclusion, these findings suggest that dietary supplementation with polyphenolic-rich foods (lentil, citrus fruit and/or red wine) may inspire new pharmacologic and dietetic therapeutic approaches for treatment of hypertension and diabetes.