Wild Passiflora (Passiflora spp.) seed oils and their nanoemulsions induce proliferation in HaCaT keratinocytes cells

• Published in: Journal of drug delivery science and technology Vol. 67; p. 102803
• Main Authors: Lôbo de Souza, Myla, Dourado, Douglas, Pinheiro Lôbo, Ivon, Couto Pires, Vinícius, Nunes de Oliveira Araújo, Sara, de Souza Rebouças, Juliana, Costa, Ana Maria, Pinho Fernandes, Caio, Machado Tavares, Natalia, de Paula Pereira, Neila, Rocha Formiga, Fabio
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 01-01-2022
• Subjects: Cell proliferation; Cytotoxicity; Herbal oils; Kinetic stability; Passion fruit; Passion fruit; Cell proliferation; Kinetic stability; Cytotoxicity; Herbal oils
• ISSN: 1773-2247
• DOI: 10.1016/j.jddst.2021.102803

Despite the potential of Passiflora spp. as potential as source of bioactive compounds, poor aqueous solubility and stability issues restrict their use in pharmaceutical field. The most suitable approach to overcome such limitations is the development of formulations based on proper delivery systems. Among them, nanoemulsions have been widely used as carrier systems that can guarantee better cutaneous performance of herbal oils. This study evaluated physicochemical properties and cellular effects of seed oils from four varieties of wild Passiflora spp. The fatty acids profile of the seed oils was analyzed by gas chromatography and cell viability assay was performed on J774 macrophages. The nanoemulsions were prepared for the different seeds oils and cell proliferation was evaluated on HaCaT keratinocytes. The oils presented a predominance of unsaturated fatty acids, mainly linoleic acid (>65%) and no cytotoxic effects were observed on J774 cells up to 100 μg/mL. All nanoemulsions presented droplet size about 200 nm, narrow polydispersity index (<0.300) and overall, physicochemical properties consistent with good stability after 90 days of storage. The oils and nanoemulsions induced proliferation of keratinocytes, where nanoemulsions prepared with P. alata oil was the most effective, reaching 150% effect. These findings demonstrate proper cytocompatibility of oils from wild Passiflora spp. and their ability to stimulate the proliferation of keratinocytes, which suggest their potential use as herbal bioactives for dermal healing. Their incorporation in nanoemulsions is an innovative approach for obtaining nanotechnological dermatological products through a sustainable concept that can reach organoleptic characteristics superior to the in natura product.
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•Oils from different Passiflora sp seeds have significant pharmaceutical potential.•The wild varieties studied herein present a high content of omega 6 and 9.•P. alata nanoemulsion highlights the feasibility of these colloids for these oils.•Both oils and nanoemulsions induced proliferation of HaCaT keratinocytes.•Obtained prototypes may be further applied on nanopharmaceuticals/cosmeceuticals.