Light Sheet Fluorescence Microscopy Optimized for Long-Term Imaging of Arabidopsis Root Development

Light Sheet Fluorescence Microscopy Optimized for Long-Term Imaging of Arabidopsis Root Development

Light sheet fluorescence microscopy (LSFM) allows sustained and repeated optical sectioning of living specimens at high spatial and temporal resolution, with minimal photodamage. Here, we describe in detail both the hardware and the software elements of a live imaging method based on LSFM and optimi...

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Bibliographic Details
Published in:Methods in molecular biology (Clifton, N.J.) Vol. 1761; p. 145
Main Authors: Baesso, Paolo, Randall, Ricardo S, Sena, Giovanni
Format: Journal Article
Language:English
Published: United States 2018
Subjects:
Arabidopsis - cytology
Arabidopsis - growth & development
Arabidopsis - metabolism
Germination
Image Processing, Computer-Assisted
Imaging, Three-Dimensional
Microscopy, Fluorescence - instrumentation
Microscopy, Fluorescence - methods
Molecular Imaging - instrumentation
Molecular Imaging - methods
Plant Development
Plant Roots - cytology
Plant Roots - growth & development
Plant Roots - metabolism
Seedlings
Software
Morphodynamics
Tracking
Live imaging
Arabidopsis root
Microscopy
Control software
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Description
Summary:Light sheet fluorescence microscopy (LSFM) allows sustained and repeated optical sectioning of living specimens at high spatial and temporal resolution, with minimal photodamage. Here, we describe in detail both the hardware and the software elements of a live imaging method based on LSFM and optimized for tracking and 3D scanning of Arabidopsis root tips grown vertically in physiological conditions. The system is relatively inexpensive and with minimal footprint; hence it is well suited for laboratories of any size.
ISSN:1940-6029
DOI:10.1007/978-1-4939-7747-5_11