Impact of vitamin D supplementation on modulating heat-shock proteins in postmenopausal women: a randomized, double-blind, placebo-controlled study

• Published in: Menopause (New York, N.Y.) Vol. 30; no. 7; pp. 758 - 765
• Main Authors: Orsatti, Claudio Lera, Orsatti, Fábio Lera, de Souza, João Pedro Esteves Alves, Nahas, Eliana Aguiar Petri
• Format: Journal Article
• Language: English
• Published: United States Lippincott Williams & Wilkins 01-07-2023
• Subjects: Cholecalciferol; Dietary Supplements; Double-Blind Method; Female; Heat-Shock Proteins; HSP27 Heat-Shock Proteins - therapeutic use; Humans; Postmenopause; Vitamin D; Vitamin D Deficiency
• ISSN: 1072-3714; 1530-0374
• DOI: 10.1097/GME.0000000000002197

The aim of this study was to examine the effect of vitamin D (VitD) supplementation on serum heat-shock proteins (HSP) in postmenopausal women (PW).
In this double-blind, placebo-controlled trial, 160 PW aged 45 to 65 years with amenorrhea 12 months or more were randomized into two groups: 80 PW in VitD group (oral supplementation with 1,000 IU VitD 3 /d) or 80 PW in placebo group. The PW were assessed at baseline and after 10 months of intervention. Plasma concentrations of 25-hydroxyVitD (25[OH]D) were measured by high-performance liquid chromatography. HSP27/pS78/pS82, HSP27/total, HSP60, HSP70/72, and HSP90α levels were assessed in serum using a multiplexed bead immunoassay.
HSP27 (pS78/pS82 [ P < 0.035] and total [ P < 0.001]) levels increased in the supplemented group when compared with the control group. There was no effect of VitD supplementation on HSP60, HSP70/72, and HSP90α levels.
Vitamin D supplementation increases serum HSP27 level in PW.